2009 Fiscal Year Final Research Report
Development of a Time-Resolved Infrared Spectrophotometer ～ Elucidation of the proton pumping mechanism ～
| Project/Area Number |
20770125
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | University of Hyogo |
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Principal Investigator |
YAMAGUCHI Satoru University of Hyogo, 大学院・生命理学研究科, 助教 (20347529)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 時間分解測定 / 赤外分光 / プロトンポンプ |
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| Research Abstract |
A femtosecond infrared laser was combined to our infrared spectrometer for light source. A chamber was constructed to cover while optical system because the water vapor makes IR spectra worth remarkably and it was filled with dry N_2 gas. For time-resolved IR experiment, a micro flow cell that dimension has 50 x 10^<-6> m (light path length) and 400 x 10^<-6> m (flow pass width) was made by sandwiched SU-8 polymer between CaF_2 windows. A difference spectra between reduced state and oxidized state of cytochrome c oxidase was acquired by using these system.
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