2010 Fiscal Year Final Research Report
The study on reaction mechanisms and dynamics of ribosome using deuterium exchange and mass spectrometry
| Project/Area Number |
20770129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YAMAMOTO Tatsuya The Institute of Physical and Chemical Research, 医学部, 助教 (70437573)
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| Project Period (FY) |
2008 – 2010
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| Keywords | タンパク質 / 核酸の構造 / 動態 / 機能 |
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| Research Abstract |
Protein synthetic process by ribosome (translation) had never been observed the detail movement in solution, because ribosome is a macro complex (2.3MDa) comprised of 55 proteins and three rRNAs. In this study, we succeeded in the simultaneous detection of 52 ribosomal proteins in solution on the working time scale by using deuterium exchange and mass spectrometry, and elucidated the magnitude of the conformation change during the translocation process in protein synthesis.
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