2009 Fiscal Year Final Research Report
Analysis on the initiation complex and a DiaA-regulating factor
| Project/Area Number |
20770139
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
KEYAMURA Kenji Kyushu University, 薬学研究院, 助教 (70464386)
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| Project Period (FY) |
2008 – 2009
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| Keywords | DNA複製 / 複製開始複合体 / タンパク質相互作用 |
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| Research Abstract |
In many cellular organisms, chromosomal replication initiation requires the regulated formation of dynamic higher-order complexes. E. coli DnaA-DiaA complexes form a specific multimer on oriC, resulting in DNA unwinding and DnaB helicase loading. DiaA, a DnaA-binding protein, directly stimulates the formation of ATP-DnaA multimers on oriC and ensures timely replication initiation. In this study, the structural model of DnaA-DiaA complex was determined. Moreover, a DiaA-regulating factor was searched.
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