2009 Fiscal Year Final Research Report
Mooecular mechanism of protein ubiquitylation, regulated by replication factor, PCNA.
| Project/Area Number |
20770140
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | University of Hyogo |
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Principal Investigator |
YASUSHI Shiomi University of Hyogo, 大学院・生命理学研究科, 助教 (80380567)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 細胞周期 / DNA複製 / ユビキチン化 / タンパク質分解 / PCNA |
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| Research Abstract |
(1) We have constracted baculovirus expression system of CRL4^<Cdt2> ubiqutin ligase, and in vitro ubiquitylation system for Cdt1, p21.(2) We have demonstrated that two conserved amino acid residues within and downstream of the PIP-box are required for degradation in mammalian cells. TD amino acid residues in the PIP-box, and the presence of basic amino acids at +3 and +4 downstream of the PIP box create a degron for CRL4^<Cdt2>. (3) We have analyzed the proteins which interacts with CRL4^<Cdt2> in vivo by mass spectrometry, and found candidates for ubiquitylation by CRL4^<Cdt2>.
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