Biochemical and genetic analysis of initiation of eukaryotic genomic DNA replication

2009 Fiscal Year Final Research Report

• Project/Area Number: 20770144
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: YAGURA Masaru National Institute of Genetics, 細胞遺伝研究系, 研究員 (00455205)
• Project Period (FY): 2008 – 2009
• Keywords: DNA複製開始機構 / 染色体DNA / 出芽酵母 / 生化学 / 遺伝学

Research Abstract

In eukaryotes, many initiation factors are assembled and disassembled on chromatin, and one of the factor, Mcm10, was thought to be involved in important role for the complex formation. I carefully assessed how Mcm10 participates in DNA replication. A thermosensitive allele of MCM10, mcm10-1 significantly reduced DNA replication at the nonpermissive condition after release from G1 block. Biochemical analysis revealed that budding yeast Mcm10 is a long and narrow shape and form a monomer, and it can bind to some DNA replication initiation factors directly.

Research Products

• [Journal Article] Ctf4 coordinates the progression of helicase and DNA polymerase alpha. (2009)
  • Author(s): Tanaka H, Katou Y, Yagura M, Saitoh K, Itoh T, Araki H, Bando M, Shirahige K
  • Journal Title: Genes Cells. 14(7) Pages: 807-20
  • Peer Reviewed
• [Presentation] 出芽酵母染色体DNA複製におけるMCM10の機能解析 (2009)
  • Author(s): 矢倉勝、荒木弘之
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 信州大学松本キャンパス、長野
  • Year and Date: 20090000
• [Presentation] 出芽酵母染色体DNA複製におけるMCM10の機能解析 (2009)
  • Author(s): 矢倉勝、荒木弘之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 20090000
• [Presentation] Ctf4, a component of the Replisome Progression Complex, links helicase and DNA polymerase alpha (2008)
  • Author(s): uki KATOU, Hirokazu TANAKA, Masashige BANDO, Masaru YAGURA, Ryu-suke NOZAWA, Takehiko ITOH, Chikashi OBUSE, Hiroyuki ARAKI, Katsuhiko SHIRAHIGE
  • Organizer: 3R symposium
  • Place of Presentation: Kakegawa, Shizuoka, Japan.
  • Year and Date: 20080000
• [Presentation] FUNCTIONS OF MCM10 IN CHROMOSOMAL DNA REPLICATION IN BUDDING YEAST (2008)
  • Author(s): Masaru YAGURA, Hiroyuki ARAKI
  • Organizer: EUKARYOTIC DNA REPLICATION AND GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, New York, USA
  • Year and Date: 20080000
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/MicGen/index.html