Planar cell polarity of multi-ciliated ependymal cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20770182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagoya City University
• Principal Investigator: HIROTA Yuki Nagoya City University, 大学院・医学研究科, 助教 (00453548)
• Project Period (FY): 2008 – 2010
• Keywords: 細胞分化

Research Abstract

Motile cilia generate constant fluid flow over epithelial tissue, and thereby influence diverse physiological processes. Such functions of ciliated cells depend on the planar polarity of the ciliated cells. Here we report that basal bodies move in the apical cell membrane during differentiation to accumulate in the anterior region of ependymal cells that line the surface of brain ventricles. Moreover, we show that non-muscle myosin II is a key regulator of this distribution of basal bodies.

Research Products

• [Journal Article] Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. (2011)
  • Author(s): Sawamoto, K., Hirota, Y., Alfaro-Cervello, C., Soriano-Navarro, M., He, X., Hayakawa-Yano, Y., Yamada, M., Hikishima, K., Tabata, H., Iwanami, A., Nakajima, K., Toyama, Y., Itoh, T., Alvarez-Buylla, A., Garcia-Verdugo, J.M., Okano, H.
  • Journal Title: J Comp Neurol (K.Sawamoto, Y.Hirota, C.Alfaro-Cervello, M.Soriano-Navarro contributed equallyto this work) 519(4) Pages: 690-713
  • Peer Reviewed
• [Journal Article] Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Journal Title: Development 137(18) Pages: 3037-3046
  • Peer Reviewed
• [Journal Article] Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia. (2010)
  • Author(s): Guirao, B., Meunier, A., Mortaud, S., Aguilar, A., Corsi, J.M., Strehl, L., Hirota, Y., Desoeuvre, A., Boutin, C., Han, Y.G., Mirzadeh, Z., Cremer, H., Montcouquiol, M., Sawamoto, K., Spassky, N.
  • Journal Title: Nat.Cell Biol. 12 Pages: 341-350
  • Peer Reviewed
• [Journal Article] Expression and proliferation-promoting role of Diversin in the neuronally committed precursor cells migrating in the adult mouse brain. (2010)
  • Author(s): Ikeda, M., Hirota, Y., Sakaguchi, M., Yamada, O., Kida, Y.S., Ogura, T., Otsuka, T., Okano, H., Sawamoto, K.
  • Journal Title: Stem Cells 28(11) Pages: 2017-2026
  • Peer Reviewed
• [Journal Article] Efhc1 deficiency causes spontaneous myoclonus and increased seizure susceptibility. (2009)
  • Author(s): Suzuki, T., Miyamoto, H., Nakahari, T., Inoue, I., Suemoto, T., Jiang, B., Hirota, Y., Itohara, S., Saido, TC., Tsumoto, T., Sawamoto, K., Hensch, TK., Delgado-Escueta, AV., Yamakawa, K.
  • Journal Title: Hum.Mol.Genet. 18 Pages: 1099-1109
  • Peer Reviewed
• [Journal Article] Various facets of vertebrate cilia : motility, signaling, and role in adult neurogenesis. (2009)
  • Author(s): Huang, S., Hirota, Y., Sawamoto, K.
  • Journal Title: Proceedings of the Japan Academy, Ser. B85 Pages: 324-336
  • Peer Reviewed
• [Presentation] Planar polarity of multiciliated ependymal cells on the lateral ventricular wall of mouse brain regulated by non-muscle myosin II (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Planar cell polarity of multiciliated ependymal cells regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Planar polarization of ependymal cells on the ventricular wall of mouse lateral ventricles. (2009)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, YS., Sakaguchi, M., Nakaya, M., Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] マウス発達期における脳室上衣細胞繊毛の平面極性獲得機構 (2009)
  • Author(s): 廣田ゆき, 黄詩惠, 澤本和延
  • Organizer: 第2回日本水頭症脳脊髄液学会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-14
• [Presentation] Motility analysis with nm-accuracy and high temporal resolution of mice ependymal cilia by confocal imaging. (2009)
  • Author(s): Shimozawa, T., Hirota, Y., Sawamoto, K., Higuchi, H.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-01
• [Presentation] Polarization of ependymal cilia required for fluid flow in the adult mouse lateral ventricles. (2009)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, Y.S., Sakaguchi, M., Nakaya, M., Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto.
  • Organizer: Construction and Reconstruction of the Brain
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-09
• [Presentation] Planar polarity decisions for directional beating of ependymal cilia and fluid flow in the adult mouse lateral ventricles. (2009)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, YS., Sakaguchi, M., Nakaya, M., Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto, K.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Polarization of ependymal cilia during mouse brain development. (2008)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, Y.S., Sakaguchi, M., Nakaya, M.,Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto, K.
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京
  • Year and Date: 2008-11-01
• [Book] Neurogenesis in the adult brain. (Neuronal Migration in The Adult Brain.) In press.
  • Author(s): Sawada, M., Huang, S., Hirota, Y., Kaneko, N., Sawamoto, K.
  • Publisher: Springer