2009 Fiscal Year Final Research Report
Development of a temporally regulable gene expression system in ascidian embryos
| Project/Area Number |
20770183
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
WADA Shuichi Nagahama Institute of Bio-Science and Technology, バイオサイエンス学部, 講師 (20378607)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 遺伝子発現調節 / 熱ショック応答 / 転写制御領域 / 誘導発現系 |
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| Research Abstract |
So far there was no inducible gene expression system in the ascidian Ciona intestinalis. We developed a heat shock-inducible gene expression system based on the cis-regulatory region of the Ciona HSP70 family gene Ci-HSPA1/6/7-like. This system allowed temporally controlled induction of gene expression in ascidian embryos. We also showed that heat shock response of Ci-HSPA1/6/7-like is mediated by the heat shock transcription factor.
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