Verification of adaptive evolution in response to changes in nitrogen availability

2010 Fiscal Year Final Research Report

• Project/Area Number: 20770194
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Chubu University
• Principal Investigator: AICHI Makiko Chubu University, 応用生物学部, 講師 (00340208)
• Project Period (FY): 2008 – 2010
• Keywords: 機能進化 / 遺伝子発現制御 / 富栄養化 / 水環境 / 環境適応

Research Abstract

Carnivorous plants grow in low-nutrient wetlands, but as a result of eutrophication due to the combustion of fossil fuels and agricultural fertilization, habitat area is reduced. We analyzed NO3- concentrations, pH and electrical conductivity of interstitial water collected from areas inhabited by Drosera routundifolia (Dr) and D. tokaiensis (Dt), which is a hybrid between Dr and D. spatulata (Ds). These results showed that Dr grows in oligotrophic conditions, and Dt grows in eutrophic conditions. Dt, but not Dr, could grow in both oligotrophic and eutrophic conditions and has the ability to adapt to both conditions. In this study, we also raised these 3 species of Drosera in a greenhouse. Dt, but not Dr, was able to grow in oligotrophic and eutrophic conditions and has the ability to adapt to both oligotrophic and eutrophic conditions. Further analysis of accumulation of NO_2, which is the toxic and reduced form of nitrate, following culturing in 5 mM NO_3- medium showed that Dr accumulated more NO_2-than Ds or Dt. We are now characterizing the structure of nitrate reductase of the 3 Drosera species.

Research Products

• [Journal Article] Regulation of nitrate assimilation in cyanobacteria. (2011)
  • Author(s): Ohashi Y, Shi W, Takatani N, Aichi M, Maeda S, Watanabe S, Yoshikawa H, Omata T.
  • Journal Title: J Exp Bot. 62(4) Pages: 1411-1424
  • Peer Reviewed
• [Journal Article] 2007-2009年に構築したハルリンドウHSl モデルの年間比較 (2011)
  • Author(s): 味岡ゆい, 愛知真木子, 上野薫, 寺井久慈, 南基泰, 米村惣太郎, 那須守, 横田樹広, 小田原卓郎
  • Journal Title: 環境アセスメント学会誌 9(1) Pages: 64-72
  • Peer Reviewed
• [Journal Article] 食虫植物トウカイコモウセンゴケとその両親の話 (2009)
  • Author(s): 愛知真木子, 市橋恭範, 南基泰
  • Journal Title: 中部大学「アリーナ」 第7号 Pages: 389-396
• [Presentation] 東海丘陵要素トウカイコモウセンゴケとその両親種の硝酸同化系遺伝子の解析 (2011)
  • Author(s): 豊田歩, 井原邦夫, 上坂一馬, 上野薫, 南基泰, 小俣達男, 小田原卓郎, 那須守, 米村惣太郎, 横田樹広, 愛知真木子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-20
• [Presentation] 東海丘陵要素トウカイコモウセンゴケの窒素応答性は母親種であるコモウセンゴケに類似している (2010)
  • Author(s): 豊田歩・市橋泰範・吉村久・中辰元・上野薫・南基泰・小俣達男・小田原卓郎・那須守・米村惣太郎・横田樹広・愛知真木子
  • Organizer: 植物学会(p.225)
  • Place of Presentation: 春日井
  • Year and Date: 20100909-20100911
• [Presentation] Network of gene regulation involved in the response of the cyanobacterium (2010)
  • Author(s): Makiko Aichi Hideo Iwasaki, Takumi Ohishi, Takao Kondo, Mamoru Sugita, Kazuo Nagai, Tatsuo Omata.
  • Organizer: Synechococcus elongatus to nitrogen limitation Nitrogen(P59)
  • Place of Presentation: Inuyama
  • Year and Date: 20100726-20100730
• [Presentation] Post-translational regulation of the cyanobacterial nitrate reductase involves the FeS center-binding domain of the enzyme. (2010)
  • Author(s): Yoshitake Ohashi, Nobuyuki Takatani, Makiko Aichi, Shin-ichi Maeda, Satoru Watanabe, Hirofumi Yoshikawa, Tatsuo Omata.
  • Organizer: Nitrogen(P64)
  • Place of Presentation: Inuyama
  • Year and Date: 20100726-20100730
• [Presentation] Evaluation of environmental factors for the conservation of genetic diversity in Gentiana thunbergii, the SATOYAMA indicator species. (2010)
  • Author(s): Yui Ajioka・Makiko Aichi・Kaoru Ueno・Hisayoshi Terai・Motoyasu Minami・Takurou Odawara・Mamoru Nasu・Shigehiro Yokota・Soutarou Yonemura.
  • Organizer: 2nd International Conference of Urban Biodiversity and Design(URBIO2010).(p.343)
  • Year and Date: 20100518-20100522
• [Presentation] Evaluation of Species Diversity and Potential Habitats of the SATOYAMA Indicator Species in Toki-Shonai River Basin. (2010)
  • Author(s): Shigehiro Yokota・Takurou Odawara・Mamoru Nasu・Soutarou Yonemura・Motoyasu Minami・Makiko Aichi・Kaoru Ueno・Hisayoshi Terai.
  • Organizer: 2nd International Conference of Urban Biodiversity and Design (URBIO2010).(p.276)
  • Year and Date: 20100518-20100522
• [Presentation] 東海丘陵要素植物トウカイコモウセンゴケとその両親種における窒素感受性の違いについて (2010)
  • Author(s): 豊田歩, 市橋泰範, 近藤香苗, 中辰元, 吉村久, 上野薫, 南基泰, 小俣達男, 小田原卓郎, 那須守, 米村惣太郎, 横田樹広, 愛知真木子
  • Organizer: 植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] 交雑起源種トウカイコモウセンゴケは,両親種より富栄養耐性である (2008)
  • Author(s): 愛知真木子・市橋泰範・小林明広・近藤香苗・吉村久・上野薫・永井和夫・南基泰・小俣達男
  • Organizer: 日本植物学会第72回年会
  • Place of Presentation: 高知
  • Year and Date: 20080925-20080927