2009 Fiscal Year Final Research Report
Molecular dissection of 35S promoter-specific DNA methylation in gentian.
| Project/Area Number |
20780005
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
MISHIBA Kei-ichiro Osaka Prefecture University, 生命環境科学研究科, 助教 (70390888)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 育種学 / 植物分子育種 / 花卉 / 発現抑制 / DNAメチル化 |
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| Research Abstract |
This study is intended to determine a causal factor for 35S promoter sequence-specific silencing in gentian. As a consequence of the analysis of nuclear factors in gentian, we found two regions in the 35S promoter that showed specific binding to nuclear factors of gentian. These two regions were consistent with the highly de novo methylated regions in the transgenic gentian plants. Since the two regions share common motifs, the gentian nuclear factors may involve the de novo methylation of the two regions.
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