2010 Fiscal Year Final Research Report
Establishment of plant regeneration system from endosperm-derived cultures and analysis for endosperm development and regeneration by analyzing imprinting genes
| Project/Area Number |
20780016
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Hokkaido University |
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Principal Investigator |
HOSHINO Yoichiro Hokkaido University, 北方生物圏フィールド科学センター, 助教 (50301875)
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| Project Period (FY) |
2008 – 2010
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| Keywords | クロミノウグイスカグラ / ハスカップ / 胚乳 / インプリント遺伝子 / 倍数性 / 培養 |
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| Research Abstract |
In this study, the novel culture procedure using endosperm has been developed in Lonicera caerulea. The cultured endosperm could regenerate into plantlets. The regenerated plants showed same ploidy level with endosperm. This means that endosperm culture is novel procedure for producing polyploid plants. To analyze regeneration ability from endosperm, genome imprinting has been focused. In this study, one of imprint genes, fertilization independent endosperm (FIE) was sequenced in the endosperm of L. caerulea. The regeneration machinery might be clarified by analyzing expression patterns of imprint genes during endosperm culture.
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