Study on inhibitory effects of plant KELP proteins interacting with virus movement proteins on virus infection

2009 Fiscal Year Final Research Report

• Project/Area Number: 20780030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: SASAKI Nobumitsu Tokyo University of Agriculture and Technology, 学術研究支援総合センター, 助教 (70431971)
• Project Period (FY): 2008 – 2009
• Keywords: 植物ウイルス / 細胞間移行 / ウイルス抵抗性 / 移行タンパク質 / 転写活性化補助因子 / 阻害因子

Research Abstract

In order to develop resistance plants for virus attacks, it is necessary to elucidate at molecular levels how inherent plant resistance genes act. In this study, we performed functional analyses of KELP genes, which had been shown to restrict virus infection spread. Our results have suggest that KELPs, conserved in a wide range of plant species, interact with a viral factor (movement protein) from at least two different viruses, interfere with their innate function, and consequently restrict virus spread.

Research Products

• [Journal Article] Interference with cell-to-cell movement of Tomato mosaic virus by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species (2010)
  • Author(s): Nobumitsu Sasaki, Tatsuro Odawara, Masakazu Deguchi, Yasuhiko Matsushita, Hiroshi Nyunoya.
  • Journal Title: Journal of General Plant Pathology 76 Pages: 69-73
  • Peer Reviewed
• [Journal Article] Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N. (2009)
  • Author(s): Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Seisuke Mimori, Jun-Shan Gao, Hiroshi Nyunoya.
  • Journal Title: Physiological and Molecular Plant Pathology 73 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement. (2009)
  • Author(s): Nobumitsu Sasaki, Takuya Ogata, Masakazu Deguchi, Shoko Nagai, Atsushi Tamai, Tetsuo Meshi, Shigeki Kawakami, Yuichiro Watanabe, Yasuhiko Matsushita, Hiroshi Nyunoya.
  • Journal Title: Molecular Plant Pathology 10 Pages: 161-173
  • Peer Reviewed
• [Presentation] Arabidopsis thaliana KELPホモログの一過的過剰発現によるTomato mosaic virusの細胞間移行に対する抑制効果 (2009)
  • Author(s): 小田原達郎、佐々木信光、丹生谷博
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Interference with Tomato mosaic virus cell-to-cell movement by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species (2009)
  • Author(s): Tatsuro Odawara, Hiroshi Nyunoya, Nobumitsu Sasaki.
  • Organizer: The 2009 KSPP Fall Meeting and the 1st Japan-Korea Joint Symposium
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-10-29
• [Presentation] Study on the regulation of the elicitor-responsive expression of Tobacco mosaic virus resistance gene N. (2009)
  • Author(s): Md. Ashraful Haque, 佐々木信光, 鐘ヶ江弘美, 高俊山, 丹生谷博
  • Organizer: 動物と植物の免疫系:その共通性と多様性.
  • Place of Presentation: 明治大学
  • Year and Date: 2009-09-14
• [Presentation] Elicitor-responsive 20-bp element of the Tobacco mosaic virus resistance gene N. (2009)
  • Author(s): Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Junshan Gao, Hiroshi Nyunoya
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec City, Canada
  • Year and Date: 2009-07-19
• [Presentation] KELPおよびKELP類似遺伝子のウイルス感染抑制効果縦軸:トマトモザイクウイルスの細胞間移行率横軸:共導入したKELPおよび類似遺伝子KELPホモログの一過的過剰発現によるトマトモザイクウイルスの細胞間移行に対する抑制効果 (2009)
  • Author(s): 小田原達郎・佐々木信光・丹生谷博.
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-03-27
• [Presentation] Identification of a cis regulatory element of the Tobacco mosaic virus resistance gene N. (2008)
  • Author(s): Md. Ashraful Haque, Nobumitsu Sasaki, Hiroshi Nyunoya
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-10
• [Presentation] Overexpression of putative transcriptional coactivator KELP interferes with virus movement (2008)
  • Author(s): Nobumitsu Sasaki, Takuya Ogata, Masakazu Deguchi, Shoko Nagai, Atsushi Tamai, Tetsuo Meshi, Shigeki Kawakami, Yuichiro Watanabe, Yasuhiko Matsushita, Hiroshi Nyunoya
  • Organizer: 9th International Congress of Plant Pathology
  • Place of Presentation: International Society for Plant Pathology. Torino, Italy
  • Year and Date: 2008-08-27
• [Presentation] Two novel N-like genes encoding functionally competent domains to induce hypersensitive response (2008)
  • Author(s): Jun-Shan Gao, Nobumitsu Sasaki, Hiromi Kanegae, Kenichi Konagaya, Kaori Takizawa, Naomi Hayashi, Yosuke Okano, Masahiro Kasahara, Yasuhiko Matsushita, Hiroshi Nyunoya.
  • Organizer: 9th International Congress of Plant Pathology
  • Place of Presentation: International Society for Plant Pathology. Torino, Italy
  • Year and Date: 2008-08-27
• [Presentation] KELPの一過的過剰発現によるトマトモザイクウイルス移行タンパク質の細胞内局在の変化 (2008)
  • Author(s): 佐々木信光、小賀田拓也、松下保彦、丹生谷博.
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 島根
  • Year and Date: 2008-04-27
• [Remarks]
  • URL: http://www.tuat.ac.jp/~idenshi/Japanese%20Files/Kenkyu_Folder/kenkyu2.html