2009 Fiscal Year Final Research Report
Yeast two-hybrid study of BmMLV protein and host protein interactions
| Project/Area Number |
20780035
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | Utsunomiya University |
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Principal Investigator |
IWANAGA Masashi Utsunomiya University, 農学部, 准教授 (40400717)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ウイルス / 培養細胞 / 持続感染 / カイコ / 感染性クローン / 相互作用 / ヘリカーゼ / two-hybrid |
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| Research Abstract |
Bombyx mori macula-like virus (BmMLV) persistently infects to B. mori-derived cell lines. However, the mechanism of virus replication is unknown. To clarify this, yeast two-hybrid screening was performed and showed that the interaction of BmMLV HELICASE and several translational factor of B. mori. Also, the screening showed that the dimerization of BmMLV HELICASE. In addition, we developed an infectious full-length cDNA clone of BmMLV.
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