2009 Fiscal Year Final Research Report
Mechanisms of toxicity of anti-insect proteins from latex of mulberry trees known as a host plant of silkworms.
| Project/Area Number |
20780040
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
WASANO Naoya National Institute of Agrobiological Sciences, 昆虫-昆虫・植物間相互作用研究ユニット, 特別研究員 (00469850)
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| Project Period (FY) |
2008 – 2009
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| Keywords | クワ / 乳液 / MLX56 / 耐虫性 / シロイヌナズナ / 囲食膜 / エリサン / カイコ |
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| Research Abstract |
A mlx56 gene encoding mulberry anti-insect protein was introduced into Arabidopsis thaliana in order to elucidate the possibility of application use of a mlx gene. The expression of mlx56 gene in leaves was observed at a transcription level, however, the MLX56 protein could not be detected by immunoblot analysis, and no toxicity was observed against larvae of the Eri-silkworms and the cabbage moth. The MLX56 bind at entire part of peritrophic membrane of larvae of the Eri-silkworm whereas little binding property was observed against that of larvae of the silkworm. The results strongly suggested that the target site of the MLX56 is the peritrophic membrane, and larvae of the silkworm circumvent from the MLX56 by developing low-affinity peritrophic membrane with the MLX56.
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[Journal Article] A unique latex protein, MLX56, defends mulberry trees from insects.2009
Author(s)
Wasano, N., Konno, K., Nakamura, M. Hirayama, C., Hattori, M., Tateishi, K.
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Journal Title
Phytochemistry 70(7)
Pages: 880-888
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[Presentation] A unique latex protein, MLX56, with chitin-binding and extensin domains defends mulberry trees from insects.2009
Author(s)
Wasano, N., Konno, K., Nakamura, M., Hirayama, C., Hattori, M., Tateishi, K.
Organizer
Entomological Society of America 57th Annual Meeting
Place of Presentation
Indianapolis
Year and Date
20091213-20091216
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[Presentation] A novel latex protein confers insect resistance on mulberry trees: Involvement of the chitin-binding protein in larval toxicity in the midgut of caterpillars.2008
Author(s)
Wasano, N., Nakamura, M., Hirayama, C., Tateishi, K., Hattori, M., Konno, K.
Organizer
International Symposium of Insect Midgut Biology
Place of Presentation
China
Year and Date
20080406-20080412
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