2009 Fiscal Year Final Research Report
Symbiotic relationship between human and bifidobacteria : Understanding and exploiting the functions of bifidobacterial glycosidases.
| Project/Area Number |
20780056
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Ishikawa Prefectural University |
|---|
Principal Investigator |
KATAYAMA Takane Ishikawa Prefectural University, 生物資源環境学部, 准教授 (70346104)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Keywords | ビフィズス菌 / ヒトミルクオリゴ糖 / ムチン型糖鎖 / グリコシダーゼ |
|---|
| Research Abstract |
Breast-fed infants normally have intestinal microbiota dominated by bifidobacteria, in contrast to formula-fed infants. This selective growth has been attributed to oligosaccharides contained in human milk (HMOs). We found that several bifidobacterial strains have a unique metabolic pathway for degrading HMOs. We isolated the specific enzymes involved in this pathway, determined their properties and x-ray structures, and revealed their structure-function relationship. Also, we succeeded in synthesizing 2'-fucosyllactose, a major component of HMOs, by exploiting 1, 2-α-L-fucosidase.
|
-
-
[Journal Article] Crystal structure of GH101 endo-α-N-acetylgalactosaminidase from Bifidobacterium longum.2009
Author(s)
Suzuki, R., Katayama, T., Fushinobu, S., Kitaoka, M., Kumagai, H., Wakagi, T., Shoun, H., Ashida, H., Yamamoto, K.
-
Journal Title
J. Appl. Glycosci. 56
Pages: 105-110
Peer Reviewed
-
[Journal Article] Conversion of inverting glycoside hydrolases into catalysts for synthesizing glycosides employing a glycosynthase technology.2009
Author(s)
Kitaoka, M., Honda, Y., Fushinobu, S., Hidaka, M., Katayama, T., Yamamoto, K.
-
Journal Title
Trends in Glycoscience and Glycotechnology. 21(117)
Pages: 23-39
Peer Reviewed
-
[Journal Article] Two distinctα-L-fucosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates.2009
Author(s)
Ashida, H., Miyake, A., Kiyohara, M., Wada, J., Yoshida, E., Kumagai, H., Katayama, T., Yamamoto, K
-
Journal Title
Glycobiology 19(9)
Pages: 1010-1017
-
[Journal Article] Crystallographic and mutational analyses of substrate recognition of endo-α-N-acetylgalactosaminidase from Bifidobacterium longum.2009
Author(s)
Suzuki, R., Katayama, T., Kitaoka, M., Kumagai, H., Wakagi, T., Shoun, H., Ashida, H., Yamamoto, K. Fushinobu, S.
-
Journal Title
J. Biochem. 146(3)
Pages: 389-398
-
[Journal Article] Functions of novel glycosidases isolated from bifidobacteria.2008
Author(s)
Katayama, T., Wada, J., Fujita, K., Kiyohara, M., Ashida, H., Yamamoto, K.
-
Journal Title
J. Appl. Glycosci. 55
Pages: 101-109
-
[Journal Article] 1, 2-α-L-Fucosynthase: A glycosynthse derived from an invertingα-glycosidase with an unusual reaction mechanism.2008
Author(s)
Wada, J., Honda, Y., Nagae, M., Kato, R., Wakatsuki, S., Katayama, T., Taniguchi, H., Kumagai, H., Kitaoka, M., Yamamoto, K.
-
Journal Title
FEBS Lett. 582
Pages: 3739-3743
-
[Journal Article] Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type-1 structure.2008
Author(s)
Wada, J., Ando, T., Kiyohara, M., Ashida, H., Kitaoka, M., Yamaguchi, M., Kumagai, H., Katayama, T., Yamamoto, K.
-
Journal Title
Appl. Environ. Microbiol. 74(13)
Pages: 3996-4004
-
[Journal Article] Structural and thermodynamic analyses of solute-binding protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I.2008
Author(s)
Suzuki, R., Wada, J., Katayama, T., Fushinobu, S., Wakagi, T., Shoun, H., Sugimoto, H., Tanaka, A., Kumagai, H., Ashida, H., Kitaoka, M., Yamamoto, K.
-
Journal Title
J. Biol. Chem. 283(19)
Pages: 13165-13173
-
-
-
-
