2009 Fiscal Year Final Research Report
The regulatory system of methanol metabolism by oxygen in methylotrophic yeast Pichia methanolica.
| Project/Area Number |
20780061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
FUJIMURA Shuki Tokyo University of Agriculture, 生物産業学部, 助教 (00453803)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 環境応答 / 酸素認識 / エピジェネティクス / メチロトローフ酵母 |
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| Research Abstract |
Methanol metabolic pathway is a good model for oxygen response system as it requires and consumes oxygen. Methanol metabolism in methylotrophc yeast, Pichia methanolica a large amount of oxygen are used for methanol oxidation and for synthesis of ATP in respiratory chain. In this study the author investigated that Pichia methanolica CYC1 expression patterns. The level of PmCYC1 expression changed with methanol concentration, and CYC1 expression was induced strongly at high oxygen concentration when grown on methanol. These results showed that, this expression pattern change of PmCYC1 is specific to methanol metabolizing activity. In eukaryotes chromatin remodeling system is important in transcriptional regulation. To find out whether histone acetylation plays a role in the regulation of AOD we tested the effect of histone decelases (HDACs) activator. As a result the expression of AOD induced by Resveratrol which is a potent activator of HDAC.
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[Journal Article] Acetoaldehyde tolerance in Saccharomyces cerevisiae involves the pentose phosphate pathway and oleic acid biosynthesis.2008
Author(s)
Matsufuji, Y., Fujimura, S., Ito, T., Nishizawa, M., Miyaji, T., Nakagawa, J., Ohyama, T., Tomizuka, N. Nakagawa
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Journal Title
Peer Reviewed
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