2010 Fiscal Year Final Research Report
Elucidation of archaea-specific archaeosine modification system
| Project/Area Number |
20780062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Toyo University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2010
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| Keywords | 微生物代謝 / アーキア / tRNA / tRNA修飾酵素 |
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| Research Abstract |
The genes involving archaeosine biosynthesis were successfully disrupted, indicating that these genes are not essential for cell viability. The growth profiles of ΔqueD, Δtgt and ΔarcS mutants were almost identical with that of host strain at 85℃ (the optimum growth temperature) and 93℃ (the upper limit of growth temperature), suggesting that archaeosine could not contribute to stabilization the tertiary structure of tRNA. The queE gene disruptant showed poor cell growth at 85 and 93℃. The queC gene disruptant showed poor cell growth at 93℃.
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