2009 Fiscal Year Final Research Report
Conversion of catalytic mechanism of glycoside hydrolases
| Project/Area Number |
20780068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
OKUYAMA Masayuki Hokkaido University, 大学院・農学研究院, 助教 (00344490)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 糖質加水分解酵素 / 触媒機構 / タンパク質工学 |
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| Research Abstract |
I discovered an unique glycoside hydrolases family which contains inverting- and retaining-catalytic enzymes. This divergence was due to the simple difference of the position of catalytic residues. We attempted to convert the catalytic mechanism of these enzyme each other. However, the attempt was failed. These results indicated that two similar enzymes diverged from a common ancestor in early stage of evolution and each enzyme evolved in different ways.
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