2009 Fiscal Year Final Research Report
Functional analysis of phosphoprotein to control endosperm formation and sugar metabolism in plant seed.
| Project/Area Number |
20780069
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
HAMADA Shigeki Hokkaido University, 作物研究所米品質研究チーム, 任期付研究員 (90418608)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Keywords | 植物生化学 |
|---|
| Research Abstract |
Protein phosphorylation plays a key regulatory role in a variety of cellular processes. To better understand the function of protein phosphorylation in seed maturation, I employed a PCR-based cloning method and isolated five cDNA clones (pvcipk1-5) for protein kinases and two cDNA clones (pvcbl1 and 2) for CBLs from a cDNA library prepared from immature seeds of kidney bean (Phaseolus vulgaris L.). Yeast two-hybrid assay indicated that among the five PvCIPKs, only PvCIPK1 interacts with both PvCBL1 and PvCBL2. These results suggest that calcium-dependent protein phosphorylation-signaling via CBL-CIPK complexes occurs during seed development. Furthermore I identified heat shock protein 82 (Hsp82) and nucleooside diphosphate kinase 1 (NDPK1) as phosphoproteins in rice developing seed. The results of subcellular localization, gene expression and mutant analysis of these proteins indicated that Hsp82 and NDPK1 are involved in starch biosynthesis in plat seed.
|
-
[Journal Article] Metabolism of tuberonic acid (12-hydroxyjasmonic acid) in plants: Purification and characterization of tuberonic acid glucosyltransferase activated by wounding stress2009
Author(s)
Seto, Y., Hamada, S., Matsuura, H., Satou, C., Masuta, C., Matsushige, M., Takahashi, K., Ito, H., Matsui, H., Nabeta, K
-
Journal Title
Phytochemistry 70(3)
Pages: 370-379
-
[Journal Article] Expression and identification of the CBLs and CIPKs from immature seeds of kidney bean (Phaseolus vulgaris L. )2009
Author(s)
Hamada, S., Seiki, Y., Watanabe, K., Ozeki, T., Matsui, H., Ito, H
-
Journal Title
Phytochemistry 70(4)
Pages: 501-507
-
[Journal Article] Identification of cis-localization elements of the maize 10 kD delta-zein and their use in the targeting RNAs to specific cortical ER subdomains2009
Author(s)
Washida, H., Sugino, A., Kaneko, S., Crofts, N., Sakulsingharoj, C., Kim, D., Choi, S.B., Hamada, S., Ogawa, M., Wang, C., Esen, A., Higgins, T.J., Okita, T. W
-
Journal Title
Plant J 60(1)
Pages: 146-155
-
[Journal Article] The cytoplasmic-localized, cytoskeletal-associated RNA binding protein OsTudor-SN: evidence for an essential role in storage protein RNA transport and localization2008
Author(s)
Wang, C., Washida, H., Crofts, A.J., Hamada, S., Katsube-Tanaka, T., Kim, D., Choi, S.B., Modi, M., Shigh, S., Okita, T. W
-
Journal Title
Plant J 55(3)
Pages: 443-454
-
-
-
-
-
-
-
-
-
