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2010 Fiscal Year Final Research Report

Environmental factor for cold-adaptation of psychrophilic metalloenzyme

Research Project

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Project/Area Number 20780074
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionKobe University

Principal Investigator

TSURUTA Hiroki  Kobe University, 連携創造本部, 准教授 (20346282)

Project Period (FY) 2008 – 2010
Keywords低温活性酵素 / 好冷性細菌 / 低温適応 / X線結晶構造解析 / 触媒反応効率 / フォスファターゼ
Research Abstract

For the construction of structure leading high cold activity of psychrophilic phosphatase, the magnesium concentration during folding of this enzyme protein was an important environmental factor.

  • Research Products

    (12 results)

All 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme.2010

    • Author(s)
      M.Nakagawa, M.Ueyama, H.Tsuruta, T.Uno, K.Kanamaru, B.Mikami, H.Yamagata.
    • Journal Title

      Journal of Biological Chemistry 285

      Pages: 29797-29807

    • Peer Reviewed
  • [Journal Article] Tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick, Ixodes persulcatus.2010

    • Author(s)
      E.Isogai, H.Isogai, K.Okumura, H.Hori, H.Tsuruta, Y.Kurebayashi.
    • Journal Title

      Experimental & Applied Acarology 53(1)

      Pages: 71-77

    • Peer Reviewed
  • [Journal Article] Crystal structure of cold-active alkaline phosphatase from psychrophile Shewanella sp.2010

    • Author(s)
      H.Tsuruta, B.Mikami, T.Higashi, Y.Aizono.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 74(1)

      Pages: 69-74

    • Peer Reviewed
  • [Journal Article] 低温で機能する酵素の機能特性と構造特性、特集「化学品量産プロセスを志向する酵素研究」2008

    • Author(s)
      鶴田宏樹、相薗泰生
    • Journal Title

      BIO INDUSTRY 25, No.7

      Pages: 5-13

  • [Journal Article] The Role of Group Bulkiness in the Catalytic Activity of Psychrophile Cold-active Protein Tyrosine Phosphatase2008

    • Author(s)
      H.Tsuruta, B.Mikami, C.Yamamoto, H.Yamagata.
    • Journal Title

      FEBS Journal 275

      Pages: 4317-4328

    • Peer Reviewed
  • [Journal Article] Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917T.2008

    • Author(s)
      K.Iwamoto, H.Tsuruta, Y.Nishitani, R.Osawa.
    • Journal Title

      Systematic and Applied Microbiology 31(4)

      Pages: 269-277

    • Peer Reviewed
  • [Presentation] Structural features for adaptation of psychrophilic cold-active phosphatase2010

    • Author(s)
      H.Tsuruta, B.Mikami.
    • Organizer
      PACIFICHEM2010
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2010-12-18
  • [Presentation] Mgイオン存在下で再生したCold-active protein tyrosine phosphataseの立体構造解析2010

    • Author(s)
      鶴田宏樹、三上文三
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学、東京
    • Year and Date
      2010-03-28
  • [Presentation] 好冷菌Shewanella sp.が産生するCold-active alkaline phosphataseの結晶構造2009

    • Author(s)
      鶴田宏樹、三上文三、東哲生、相薗泰生
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド、兵庫
    • Year and Date
      2009-10-22
  • [Presentation] 配位金属の相違が低温活性発現能に与える影響2008

    • Author(s)
      鶴田宏樹
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド、兵庫
    • Year and Date
      2008-12-12
  • [Presentation] 低温活性フォスファターゼの低温での効率より機能発現に関わる要因2008

    • Author(s)
      田宏樹
    • Organizer
      極限微生物学会年会
    • Place of Presentation
      立教大学、東京
    • Year and Date
      2008-11-04
  • [Remarks] ホームページ

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Published: 2012-02-13   Modified: 2016-04-21  

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