2010 Fiscal Year Final Research Report
Construction of D-aminoacyl peptides synthetase
| Project/Area Number |
20780075
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Tottori University |
|---|
Principal Investigator |
ARIMA Jiro Tottori University, 農学部, 講師 (80393411)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | タンパク質工学 / D-アミノペプチダーゼ / アミノリシス / ペプチド合成 |
|---|
| Research Abstract |
Peptides incorporating D-amino acids are expected to increase the functional variety and applications of peptides. In this study, we constructed the mutant D-aminopeptidases as a convenient tool for synthesis of peptides incorporating D-amino acids. We obtained a serine D-stereospecific amidohydrolase that exhibit peptide bond formation activity by catalysis of aminolysis reaction. The enzyme utilizes D-Pro derivatives as acyl donor substrate, and exhibit efficient synthesis of cyclo (D-Pro-L-Arg) that has chitinase inhibitory activity in a one-pot reaction manner. In addition, we identified the residue, Asn193, which associates the recognition of side chain of acyl donor, from the mutation analysis.
|
Research Products
(10 results)
