Terpene synthases and terpene-modifying enzymes: structure, catalytic mechanism, and application.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20780078
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: University of Shizuoka
• Principal Investigator: ITO Sohei University of Shizuoka, 生活健康科学研究科, 助教 (70372836)
• Project Period (FY): 2008 – 2010
• Keywords: タンパク質工学 / X線結晶構造解析 / モノテルペン合成酵素 / セスキテルペン合成酵素 / シンナミルアルコール脱水素酵素

Research Abstract

Plants produce many thousands of terpenes for certain essential physiological roles. The enormous number and diversity of terpenes in plants is though to arise from diversities of precoursor-producing enzymes, terpene synthases and terpene-modifying enzymes. We performed identification, cloning, and characterization of these enzymes from Citrus unshiu, Camellia sinensis, etc. Especially, we determined the structure of terpene alcohol dehydrogenase complexed with substrate and coenzyme.

Research Products

• [Journal Article] Molecular cloning and characterization of a linalool synthase from lemon myrtle(accepted for publication) (2011)
  • Author(s): Sugiura, M., Ito, S., Saito, T., Niwa, Y., Koltunow, A.M., Sugimoto, O., Sakai, H.
  • Journal Title: Biosci.Biotecnol.Biochem. in press
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of geraniol dehydrogenase from Backhousia citriodora (lemon myrtle) (2011)
  • Author(s): Saito, Y., Ito, S., Koltunow, A.M., Sakai, H.
  • Journal Title: Acta Crystallogr.Sect.F Struct.Biol.Cryst.Commun. 67巻 Pages: 665-667
  • Peer Reviewed
• [Journal Article] Crystal Structure of Glucansucrase from the Dental Caries Pathogen Streptococcus mutans. (2011)
  • Author(s): Ito, K., Ito, S., Shimamura, T., Weyand, S., Kawarasaki, Y., Misaka, T., Abe, K., Kobayashi, T., Cameron, A.D., Iwata, S.
  • Journal Title: J.Mol.Biol. 408巻 Pages: 177-186
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans. (2010)
  • Author(s): Ito K, Ito S, Shimamura T, Kawarasaki Y, Abe K, Misaka T, Kobayashi T, Iwata S.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun. 66巻 Pages: 1086-1088
  • Peer Reviewed
• [Presentation] レモンマートルの5つのアルコール脱水素酵素遺伝子のクローニングとその発現酵素の特性解析 (2011)
  • Author(s): 斉藤瑛介, 伊藤創平, Anna M.Koltnow、酒井坦
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-26
• [Presentation] Gene Expression Profiling of Some Aroma Relating Enzymes in Tea Leaves (2010)
  • Author(s): Sakai, K., Fuchita, T., Ogawa, T., Kobayashi, E., Ito, S., Sakai, H.
  • Organizer: The 4th International Conference on O-CHA (Tea)
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-27
• [Presentation] Characterization of key enzymes involved in citral synthesis in Lemon myrtle (Bachhousia citriodra) (2010)
  • Author(s): Saito, Y,, Ito, S., Sugiura, M., Koltunow, A.M., Sakai, H.
  • Organizer: The 4th International Conference on O-CHA (Tea)
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-27
• [Presentation] Cloning of Monoterpene Synthase Genes from Camellia sinensis and their Expression in Young Leaves (2010)
  • Author(s): Nagumo, K., Sakai, K., Ogawa, T., Ito, S., Sakai, H.
  • Organizer: The 4th International Conference on O-CHA (Tea)
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-27
• [Presentation] 茶(Camellia sinensis)のモノテルペン合成酵素遺伝子のクローニング (2010)
  • Author(s): 南雲健太郎、杉浦瑞枝、伊藤創平、酒井坦
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] NPP依存型リモネン/3-カレン合成酵素基質特異性に関する研究 (2010)
  • Author(s): 池田大樹、杉浦瑞枝、伊藤創平、酒井坦
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] ミカン由来短鎖プレニルトランスフェラーゼのクローニングと機能解析 (2009)
  • Author(s): 杉浦瑞枝、伊藤創平、酒井坦
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] ユズ由来リモネン/3-カレン合成酵素の生産物の改変と基質特異性 (2009)
  • Author(s): 池田大樹、伊藤創平、杉浦瑞枝、鈴木康正、天野貴浩、酒井坦
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] Geranyl diphosphate synthase from Citrus unhsiu-cDNA cloning and functional expression- (2009)
  • Author(s): M.Sugiura, S.Ito, H.Sakai
  • Organizer: The 2nd Nano-Biosymposium 2009
  • Place of Presentation: Shizuoka
  • Year and Date: 2009-03-06
• [Presentation] レモンマートル由来リナロール合成酵素遺伝子のクローニングと酵素の機能解析 (2008)
  • Author(s): 杉浦瑞枝、丹羽康夫、Anna M.Koltunow, 杉本収、酒井坦、伊藤創平
  • Organizer: 第52回香料・テルペンおよび精油化学に関する討論会
  • Year and Date: 2008-11-01
• [Presentation] Cloning and characterization of a novel monoterpene synthase gene from Citrus junos. (2008)
  • Author(s): Y.Niwa, S.Ito, T.Amano, M.Sugiura, H.Kobayashi, T.Nakayama, H.Sakai
  • Organizer: Plant Biology 2008
  • Place of Presentation: Mexico
  • Year and Date: 2008-06-27
• [Patent(Industrial Property Rights)] 組換えサビネン合成酵素及びその利用 (2008)
  • Inventor(s): 酒井坦, 伊藤創平
  • Industrial Property Rights Holder: 静岡県国立大学法人
  • Industrial Property Number: 特許,特願2008-052890
  • Filing Date: 2008-03-04