2009 Fiscal Year Final Research Report
Cloning and application of cannabinoid biosynthetic genes based on EST analysis
| Project/Area Number |
20780084
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
TAURA Futoshi Kyushu University, 大学院・薬学研究院, 助教 (00301341)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 生合成 |
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| Research Abstract |
In this study, I cloned a gene encoding oli vetol synthase, the polyketide synthase that synthesizes the phenolic moiety of cannabi noids in Cannabis sativa, by means of EST analysis and RT-PCR, and then characterized the bacterially expressed recombinant oli vetol synthase to clarify the structural and functional properties in detail. In addition, I attempted to obtaina gene for the prenyltransferase that synthesize the carbon skeleton of cannabinoids, resultingincloning of two candi date genes for the prenyltransferase. Interestingly, I confirmed that these putativeprenyltransferases showmore that 50% identity with the prenyltransferase involved in secondary net abolismin hop, a close relative of.C. sative.
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