2010 Fiscal Year Final Research Report
The bacterial controls using antimicrobial peptides from complement components of fish
| Project/Area Number |
20780148
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Fukuoka Women's Junior College |
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Principal Investigator |
MUTSURO Junichi Fukuoka Women's Junior College, 食物栄養科, 講師 (40399100)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 魚病 / 生体防御 / 補体 / 抗菌ペプチド / 食品保存 |
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| Research Abstract |
Our results revealed that the synthetic peptide corresponding to partial amino acid sequence (KVFLECCNLMKTHKNMKTE) of C3a region of carp C3 isotype (C3-Q1) was antibacterial, and acted on Gram-negative bacteria, Escherichia coli. (XL1-Blue mrf and O157:H7 (VT1 and VT2)) and S.Enteritidis. We successfully enhanced antibacterial activity, by partially replacing amino acids of above-mentioned sequence. However, it was found that enhancement of antibacterial activity led to high cytotoxic activity. Hereafter, we aim to create antimicrobial peptides with low side effects on the basis of observation in this study.
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