Analysis for regulation of transcription factor FOXO1 by post-translational modification and mechanism of determination of lifspan

2009 Fiscal Year Final Research Report

• Project/Area Number: 20780237
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: DAITOKU Hiroaki University of Tsukuba, 大学院・生命環境科学研究科, 講師 (30361314)
• Project Period (FY): 2008 – 2009
• Keywords: 転写制御 / 翻訳後修飾 / 寿命 / 酸化ストレス

Research Abstract

In response to oxidative stress, the Forkhead transcription factor FOXO1 is activated and thereby promotes the expression of anti-oxidant genes. Moreover, FOXO has been shown to act as lifespan modulator in model organisms. Here we have demonstrated that post-translational modification plays an important role in regulation of FOXO1 function. Furthermore, by using genetic experiments in C. elegans, we have revealed that arginine methylation of FOXO1 is involved in the regulation of lifespan.

Research Products

• [Journal Article] Regulation of FOXO1-mediated transcription and cell proliferation by PARP-1. (2009)
  • Author(s): Sakamaki, J., Daitoku, H., Yoshimochi, K., Miwa, M., Fukamizu A.
  • Journal Title: Biochem. Biophys. Res. Commun. 382 Pages: 497-502
  • Peer Reviewed
• [Journal Article] Foxo1 increases pro-inflammatory gene expression by inducing C/EBPβin TNF-α-treated adipocytes (2008)
  • Author(s): Ito, Y., Daitoku, H., Fukamizu, A.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 290-295
  • Peer Reviewed
• [Journal Article] Arginine methylation of FOXO transcription factors inhibits their phosphorylation by Akt (2008)
  • Author(s): Yamagata, K., Daitoku, H., Takahashi, Y., Namiki, K., Hisatake, K., Kako, K., Mukai, H., Kasuya, Y., Fukamizu, A.
  • Journal Title: Mol. Cell 32 Pages: 221-231
  • Peer Reviewed
• [Presentation] Crosstalk regulation of arginine methylation and Akt-induced phosphorylation (2009)
  • Author(s): Daitoku H, Yamagata K, Takahashi Y, Sakamaki JI, Fukamizu A.
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] DNA damage control by FOXO1 in response to UV irradiation (2009)
  • Author(s): Daitoku H, Yoshimochi K, Fukamizu A.
  • Organizer: 第24回内藤コンファレンス「細胞核ダイナミクスとRNA [II]」
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-25
• [Presentation] アルギニンメチル化による転写因子FOXO1の機能制御機構 (2009)
  • Author(s): 大徳浩照
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: つくば
  • Year and Date: 2009-06-20
• [Presentation] Regulation of Foxo family by arginine methylation in response to oxidative stress (2008)
  • Author(s): Daitoku H, Yamagata K, Fukamizu A.
  • Organizer: Biochemistry and Molecular Biology (BMB2008)シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Book] 深水昭吉FOXOとアンチエイジング医学アンチエイジング医学の基礎と臨床改訂2版 (2008)
  • Author(s): 大徳浩照
  • Total Pages: 40-42
  • Publisher: メディカルビュー社
• [Remarks]
  • URL: http://akif2.tara.tsukuba.ac.jp/