2009 Fiscal Year Final Research Report
Development of a Sequence-specific detection probe of damaged nucleobase for noninvasive DNA damage imaging.
| Project/Area Number |
20790010
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
KODAMA Tetsuya Osaka University, 薬学研究科, 助教 (00432443)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 生体関連物質 / 人工核酸 / 分子認識 / 酸化損傷塩基 / 可視化 |
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| Research Abstract |
To develop a sequence-specific detection method of damaged nucleobase for noninvasive DNA damage imaging, we successfully created a novel bridged nucleic acid monomer that can recognize 8-hydroxyguanine in double-stranded DNA in a sequence-specific manner. Additionally, several nucleic acids, which convert into an oxidative lesion 5-formyluridne in double-stranded DNA in response to photo irradiation, were also synthesized. Moreover, we designed and synthesized a triplex forming oligonucleotide probe by organizing the 8-hydroxyguanine-recognizing nucleic acid monomer and acid-labile phosphoramidite linkage, and the fluorescent labeling of the probe was achieved for its noninvasive imaging use.
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[Journal Article] Design, Synthesis, and Evaluation of a Novel Bridged Nucleic Acid, 2', 5'-BNAON, with S-type Sugar Conformation Fixed by N-O Linkage.2009
Author(s)
Tetsuya Kodama, Chieko Matsuo, Hidetsugu Ori, Tetsuya Miyoshi, Satoshi Obika, Kazuyuki Miyashita, Takeshi Imanishi
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Journal Title
Tetrahedron 65
Pages: 2116-2123
Peer Reviewed
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