2009 Fiscal Year Final Research Report
Development of phosphate-affinity two-dimensional fluorescence difference gel electrophoresis
| Project/Area Number |
20790036
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
KINOSHITA Emiko Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (40379912)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ナノ材料 / 分子認識 / 蛋白質 / バイオテクノロジー / 分析化学 / リン酸化 / フォスタグ / 電気泳動 |
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| Research Abstract |
The researcher developed a novel type of two-dimensional electrophoresis using phosphate-affinity polyacrylamide gel electrophoresis (PAGE) for the analysis of phosphoproteins. By this procedure, the separations of phosphoprotein isotypes should be improved relative to those by current PAGE methods.
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[Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides2009
Author(s)
T akiyama, K., Kinoshita, E., Emiko Kinoshita-Kikuta, Fujioka, Y., Kubo, Y., Koike, T
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Journal Title
Anal. Biochem 388
Pages: 235-241
Peer Reviewed
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[Journal Article] Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes2009
Author(s)
K inoshita, E., Emiko Kinoshita-Kikuta, Matsubara, M., Aoki, Y., Ohie, S., Mouri, Y., Koike, T
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Journal Title
Electrophoresis 30
Pages: 550-559
Peer Reviewed
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[Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway2008
Author(s)
I shiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Kinoshita, E., Emiko Kinoshita-Kikuta, Koike, T., Tashiro, S., Elledge, S.J., Takata, M
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Journal Title
Nat. Struct. Mol. Biol 15
Pages: 1138-1146
Peer Reviewed
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[Journal Article] Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE2008
Author(s)
K inoshita, E., Emiko Kinoshita-Kikuta, Matsubara, M., Yamada, S., Nakamura, H., Shiro, Y., Aoki, Y., Okita, K., Koike, T
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Journal Title
Proteomics 8
Pages: 2994-3003
Peer Reviewed
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