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2010 Fiscal Year Final Research Report

Development of HSCCC/MS for the efficient purification of natural actives.

Research Project

  • PDF
Project/Area Number 20790043
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKinjo Gakuin University

Principal Investigator

KOICHI Inoue  Kinjo Gakuin University, 薬学部, 助教 (30339519)

Project Period (FY) 2008 – 2010
Keywords高速向流クロマトグラフィー / 質量分析法 / 天然機能性物質 / 単離精製
Research Abstract

This study aims to develop useful purification utilized with mass spectrometric monitoring for high-speed counter-current chromatography (HSCCC), which is free of irreversible adsorption and offers high resolution comparable to column chromatography. HSCCC has been widely used in preparative separation and purification of natural, medical and biochemical products. Our study was reported that HSCCC system was efficiently applied to separation of the gentamicin and polypeptide using on-line mass spectrometric monitoring with electrospray ionization. This technique would be useful purification of bioactive compounds for continuous mass spectrometric data acquisition, and 'target'-guided fractionation of small/large molecular. In this report, the HSCCC coupled with mass spectrometry technique was developed for the purification of bioactive compounds in natural extracts.

  • Research Products

    (9 results)

All 2011 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] Screening assay of angiotensin-converting enzyme inhibitory activity from complex natural colorants and foods using high-throughput LC-MS/MS.2011

    • Author(s)
      K.Inoue, M.Kitade, T.Hino, H.Oka
    • Journal Title

      Food Chem. 126

      Pages: 1909-1915

    • Peer Reviewed
  • [Journal Article] Efficient purification of xanthomonasin A and B from Monascus yellow colorant by high-speed countercurrent chromatography2010

    • Author(s)
      井之上浩一、伊藤有香、服部靖子、堤内要、伊藤澄夫、日野知証、岡尚男
    • Journal Title

      日本食品化学学会誌 17

      Pages: 185-191

    • Peer Reviewed
  • [Journal Article] An approach to on-line electrospray mass spectrometric detection of polypeptide antibiotics of enramycin for high-speed counter-current chromatographic separation.2010

    • Author(s)
      K.Inoue, Y.Hattori, T.Hino, H.Oka
    • Journal Title

      J.Pharm.Biomed.Anal. 51

      Pages: 1154-1160

    • Peer Reviewed
  • [Presentation] 天然由来有効成分の効率的単離精製を目指した高速向流クロマトグラフィー質量分析法の開発2011

    • Author(s)
      服部靖子, 井之上浩一, 日野知証, 岡尚男
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      20110328-20110331
  • [Presentation] 高速向流クロマトグラフィー/質量分析法によるゲンタマイシンC1, C2, C1aの単離精製およびその抗菌活性の測定2010

    • Author(s)
      服部靖子, 井之上浩一, 日野知証, 堀江正一, 岡尚男
    • Organizer
      第100回日本食品衛生学会学術講演会
    • Place of Presentation
      熊本
    • Year and Date
      20100916-20100917
  • [Presentation] Strategy of High-speed Countercurrent Chromatography Purification of Specific Antioxidants from Natural Products Based on On-Line HPLC Method with Radical Scavenging Assay2010

    • Author(s)
      Koichi Inoue, Erika Baba, Yasuko Hattori, Tomoaki Hino, Hisao Oka
    • Organizer
      The 6th International Conference on Countercurrent Chromatography
    • Place of Presentation
      Lyon, France
    • Year and Date
      20100726-20100730
  • [Presentation] 高速向流クロマトグラフィー質量分析法による動物用医薬品構成成分の効率的分離精製法2010

    • Author(s)
      服部靖子, 井之上浩一, 日野知証, 岡尚男
    • Organizer
      日本食品化学学会第16回総会・学術大会
    • Place of Presentation
      大阪
    • Year and Date
      20100609-20100610
  • [Presentation] 高速向流クロマトグラフィー/質量分析法によるゲンタマイシン類の単離精製2010

    • Author(s)
      服部靖子, 井之上浩一, 日野知証, 岡尚男
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      20100328-20100330
  • [Presentation] 高速向流クロマトグラフィー/ESI-MSによる抗菌剤エンラマイシン異性体の単離精製法の検討2009

    • Author(s)
      服部靖子、井之上浩一、日野知証、岡尚男
    • Organizer
      第34回日本医用マススペクトル学会年会
    • Place of Presentation
      近畿大学
    • Year and Date
      20090910-20090911

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Published: 2012-02-13   Modified: 2016-04-21  

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