The mechanism of endocytosis regulated by orchestrated activations of multiple small GTPases

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: KAJIHO Hiroaki The University of Tokyo, 大学院・薬学系研究科, 助教 (70401221)
• Project Period (FY): 2008 – 2009
• Keywords: エンドサイトーシス / メンブレントラフィック

Research Abstract

Exchanging materials between outer environments is essential for cells to maintain their homeostasis. Cells accomplish it by utilizing vesicular trafficking called endocytosis and exocytosis. Rab proteins are part of the Ras superfamily of small GTPases, which works switch molecules by undergoing a cycle between GDP-bound and GTP-bound form. Our research is focused on the molecular mechanism how Rab5, one of Rab family, regulates endocytosis. We have elucidated that multiple Rab proteins are activated by RIN family proteins.

Research Products

• [Journal Article] Filamin associates with stress signaling kinases MKK7 and MKK4 and regulates JNK activation. (2010)
  • Author(s): Nakagawa K, Sugahara M, Yamasaki T, Kajiho H, Takahashi S, Hirayama J, Minami Y, Ohta Y, Watanabe T, Hata Y, Katada T, Nishina H.
  • Journal Title: THE BIOCHEMICAL JOURNAL 427 Pages: 237-245
  • Peer Reviewed
• [Journal Article] CrxOS maintains the self-renewal capacity of murine embryonic stem cells. (2009)
  • Author(s): Saito R, Yamasaki T, Nagai Y, Wu J, Kajiho H, Yokoi T, Noda E, Nishina S, Niwa H, Azuma N, Katada T, Nishina H.
  • Journal Title: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 390 Pages: 1129-1135
  • Peer Reviewed
• [Journal Article] Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Arl6 causing Bardet-Biedl syndrome. (2009)
  • Author(s): Kobayashi T, Hori Y, Ueda N, Kajiho H, Muraoka S, Shima F, Kataoka T, Kontani K, Katada T.
  • Journal Title: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 381 Pages: 439-432
  • Peer Reviewed
• [Journal Article] Blockage by SP600125 of Fcepsilon receptor-induced degranulation and cytokine gene expression in mast cells is mediated through inhibition of phosphatidylinositol 3-kinase signaling pathway. (2009)
  • Author(s): Tanemura S, Momose H, Shimizu N, Kitagawa D, Seo J, Yamasaki T, Nakagawa K, Kajiho H, Penninger JM, Katada T, Nishina H.
  • Journal Title: J Biochem. 145 Pages: 345-354
  • Peer Reviewed
• [Journal Article] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles. (2008)
  • Author(s): Yoshikawa M*, Kajiho H*, Sakurai K, Minoda T, Nakagawa S, Kontani K, Katada T.
  • Journal Title: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 372 Pages: 168-172
  • Peer Reviewed
• [Presentation] 低分子量G蛋白質Rab5GEF RINファミリーによる小胞輸送の機構解析 (2010)
  • Author(s): 梶保博昭、池田誠一、福島槙一、堅田利明
  • Organizer: 第一回GPCM
  • Place of Presentation: 東京
  • Year and Date: 2010-02-07
• [Presentation] RIN3のリン酸化状態によるRab5サブファミリーへのGEF活性調節機構(WS口頭発表) (2009)
  • Author(s): 梶保博昭、堅田利明
  • Organizer: 文部科学省特定領域研究「細胞情報ネットワークを統合するg蛋白質シグナル研究の新展開」班会議
  • Place of Presentation: 南房総・富浦
  • Year and Date: 2009-09-12
• [Presentation] RINファミリーによる低分子量g蛋白質Rab31の活性化機構解析(ポスター発表) (2009)
  • Author(s): 梶保博昭、堅田利明
  • Organizer: 定量生物学の会第1回年会
  • Place of Presentation: 東京
  • Year and Date: 2009-01-11
• [Presentation] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles. (2008)
  • Author(s): Hiroaki Kajiho, Manabu Yoshikawa, Kyoko Sakurai, Tomohiro Minoda, Satoshi Nakagawa, Kenji Kontani, Toshiaki Katada [Poster]
  • Organizer: 48th ASCB Annual Meeting
  • Place of Presentation: 米国、サンフランシスコ
  • Year and Date: 2008-12-17
• [Presentation] Rab5結合蛋白質RIN3の小胞局在化機能解析[文部科学省特定領域研究 (2008)
  • Author(s): 梶保博昭、吉川学、堅田利明
  • Organizer: 細胞情報ネットワークを統合するg蛋白質シグナル研究の新展開
  • Place of Presentation: 湯沢
  • Year and Date: 2008-09-19