2009 Fiscal Year Final Research Report
Functional analysis of an ABC transporter HAF-2, which is expressed in neurons and muscles in Caenorhabditis elegans
| Project/Area Number |
20790071
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Iwate Medical University |
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Principal Investigator |
SHIRAISHI Hirohisa Iwate Medical University, 薬学部, 講師 (80393156)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 線虫 / ABC輸送体 / リソソーム / 貪食細胞 / 神経 / 筋肉 |
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| Research Abstract |
HAF-2, which is one of the nematode homologues of human lysosomal ABC (ATP-binding cassette) transporter, is expressed in neuronal cells, muscles, some of intestinal cells, and coelomocytes in Caenorhabdits elegans. We found that at least in coelomocytes, HAF-2 localizes on the lysosome-related granules and is not essential for the uptake of foreign substances. HAF-2 was also expressed in neuronal cells including amphid and phasmid sensory neurons. The deletion for the haf-2 gene showed the slight defects in growth rate, lifespan, brood size, defecation rate, and so on, suggesting that the intracellular lysosomal transporter is involved in the various physiological aspects at the organismal level.
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