2009 Fiscal Year Final Research Report

Characterization and functional analyses of Hic-5 deficient mice

Research Project

Project/Area Number	20790076
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Biological pharmacy
Research Institution	Showa University
Principal Investigator	KANEYAMA Shuri Showa University, 医学部・生化学, 講師 (10338535)
Co-Investigator(Renkei-kenkyūsha)	TAKEDA Naoki 熊本大学, 生命資源研究支援センター生物資源発研究部門技術開発分野, 助教 (90304998)
Project Period (FY)	2008 – 2009
Keywords	Hic-5 / ノックアウトマウス / 動脈硬化
Research Abstract	Hic-5(hydrogen peroxide-inducible clone-5) is a focal adhesion adaptor protein proposed as a candidate for a mediator of mechanotransduction. In the present study, we generated Hic-5-deficient mice by targeted mutation. Mice lacking Hic-5 are viable and fertile, and show no obvious histological abnormalities including vasculature. However, after wire injury of the femoral artery in Hic-5 deficient mice, histological recovery of arterial media was delayed due to enhanced apoptosis of vascular wall cells, whereas neointima formation was enhanced. Stretch-induced apoptosis was enhanced in cultured vascular smooth muscle cells from Hic-5 deficient mice.

(5 results)