2009 Fiscal Year Final Research Report
Functional analysis of chondroitin sulfate chains in cellular differentiation processes
| Project/Area Number |
20790080
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
MIKAMI Tadahisa Kobe Pharmaceutical University, 薬学部, 講師 (20330425)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 糖鎖 / 発現制御 / 発生・分化 / 細胞・組織 / 酵素 |
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| Research Abstract |
Chondroitin sulfate (CS) chains are major components of extracellular matrix in nervous system, and have a broad impact on various neurobiological phenomena. In this project, regulatory roles of CS in neuritogenesis were investigated using several model systems, and the key findings are as follows : 1) A plasma membrane-tethered cell adhesion molecule, contactin-1, can function as a neuronal cell-surface CS receptor, and mediates neurite extension promoted by an oversulfated CS variant, CS-E. 2) A morpholino-based knockdown of chondroitin 4-O-sulfotransferase-1, which contributes substantially to the in vivo construction of 4-O-sulfated CS, revealed the functional importance of the characteristic CS sulfation codes in motor axon guidance in zebrafish embryogenesis.
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[Journal Article] Chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish.2009
Author(s)
Mizumoto, S., Mikami, T., Yasunaga, D., Kobayashi, N., Yamauchi, H., Miyake, A., Itoh, N., Kitagawa, H., Sugahara, K.
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Journal Title
Biochem. J. 419(2)
Pages: 387-399
Peer Reviewed
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