Chondroitin 4-O-sulfotransferase-1 modulates Wnt-3a signaling through control of E disaccharide expression of chondroitin sulfate

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: NADANAKA Satomi Kobe Pharmaceutical University, 薬学部, 講師 (60378578)
• Project Period (FY): 2008 – 2009
• Keywords: 生化学 / 糖鎖生物学 / プロテオグリカン / コンドロイチン硫酸 / シグナル伝達

Research Abstract

Wnt signaling is implicated in numerous physiological events such as morphogenesis. Here we show the importance of chondroitin 4-O-sulfotransferase-1 (C4ST-1) in the efficient signaling of Wnt-3a. We propose a mechanism in which structural alterations in CS chains mediated by C4ST-1 control the strength of Wnt-3a signaling.

Research Products

• [Journal Article] Biosynthesis of heparan sulfate in EXT1-deficient cells (2010)
  • Author(s): Megumi Okada, Satomi Nadanaka, Naoko Shoji, Jun-ichi Tamura, Hiroshi Kitagawa
  • Journal Title: Biochemical Journal (in press)
  • Peer Reviewed
• [Journal Article] Sulfation of the galactose residues in the glycosaminoglycan-protein linkage region by recombinant human chondroitin 6-O-sulfotransferase-1. (2008)
  • Author(s): Kitagawa, H., Tsutsumi, K., Ikegami-Kuzuhara, A., Nadanaka, S., Goto, F., Ogawa, T., Sugahara, K.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 27438-27443
  • Peer Reviewed
• [Journal Article] Chondroitin 4-O-sulfotransferase-1 modulates Wnt-3a signaling through control of E disaccharide expression of chondroitin sulfate. (2008)
  • Author(s): Nadanaka S, Ishida M, Ikegami M, Kitagawa H.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 27333-27343
  • Peer Reviewed
• [Journal Article] Heparan sulphate biosynthesis and disease. (2008)
  • Author(s): Nadanaka, S., Kitagawa, H.
  • Journal Title: Journal of Biochemistry 144 Pages: 7-14
  • Peer Reviewed
• [Journal Article] 受容体結合における硫酸化グリコサミノグリカンの役割 (2008)
  • Author(s): 宮田真路、灘中里美、北川裕之
  • Journal Title: 蛋白質核酸酵素 53 Pages: 1533-1539
  • Peer Reviewed
• [Presentation] Wnt-3a-mediated down-regulation of chondroitin 4-O-sulfotransferase-1 gene modulates diffusion of Wnt-3a (2010)
  • Author(s): 木内啓貴, 池上優美, 灘中里美, 北川 裕之
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 20100328-20100330
• [Presentation] Fine-tuning of the Cellular Response to Wnt-3a by Chondroitin 4-O-sulfotransferase-1比較発生糖鎖生物学とその医工学への応用 (2009)
  • Author(s): 灘中里美, 石田美穂, 池上優美, 木内 啓貴, 北川裕之
  • Organizer: に関する日本・オーストリア二国間セミナー
  • Place of Presentation: 湘南国際村
  • Year and Date: 20090921-20090922
• [Presentation] Wnt-3a-mediated down-regulation of chondroitin 4-O-sulfotransferase-1 gene modulates diffusion of Wnt-3a (2009)
  • Author(s): 木内啓貴, 池上優美, 灘中里美, 北川 裕之
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 飛騨高山
  • Year and Date: 20090909-20090911
• [Presentation] EXT遺伝子ファミリーによるヘパラン硫酸鎖の合成 (2009)
  • Author(s): 久世彩加, 岡田めぐみ, 灘中里美, 北川裕之
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 飛騨高山
  • Year and Date: 20090909-20090911
• [Presentation] コンドロイチン-4-O-硫酸基転移酵素-1によるWnt-3aシグナリングの微細調節 (2009)
  • Author(s): 灘中里美, 石田美穂, 池上優美, 北川 裕之
  • Organizer: 第59回日本薬学会近畿支部総会・大会
  • Place of Presentation: 近畿大学
  • Year and Date: 2009-10-24
• [Presentation] コンドロイチン-4-O-硫酸基転移酵素-1によるWnt-3aシグナリングの微細調節 (2009)
  • Author(s): 灘中里美, 石田美穂, 池上優美, 木内 啓貴, 北川裕之
  • Organizer: 第56回日本生化学会近畿支部例会
  • Place of Presentation: 大阪医科大学
  • Year and Date: 2009-05-23