Mechanisms of streptococcal infection investigated by whole genomic tiling array

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: MARUYAMA Fumito The University of Tokyo, 大学院・生命理工学研究科, 助教 (30423122)
• Project Period (FY): 2008 – 2009
• Keywords: 微生物・感染症学 / タイリングアレイ

Research Abstract

Genomic contents of group A streptococci is rich in A or T nucleotides. This causes problem in determination of transcription factors and/or prediction of open reading regions. Firstly, we have established the method to analyze the transcriptome of streptococcal cells inside host eukaryotic cells with cDNA microarray and tiling array. The data obtained from the arrays revealed the existence of 58 novel non-coding RNA and importance of unknown genes to combat with host immune system. These things suggests various pathogenicities of group A streptococci are resulted from these non-coding RNA and hypothetical genes.

Research Products

• [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content (2009)
  • Author(s): F. Maruyama, M. Kobata, K. Kurokawa, K. Nishida, A. Sakurai, K. Nakano, R. Nomura, S. Kawabata, T. Ooshima, K. Nakai, M. Hattori, S. Hamada, I. Nakagawa
  • Journal Title: BMC Genomics 10 Pages: 358
  • Peer Reviewed
• [Journal Article] Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids. (2009)
  • Author(s): F. Maruyama*, T. Nozawa*, C. Aikawa, A. Sakurai, I. Nakagawa
  • Journal Title: ournal of Bioscience and Bioengineering 104 Pages: 471-473
  • Peer Reviewed
• [Journal Article] Reactive oxygen species induced by fibronectin-mediated Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells.
  • Author(s): C. Aikawa, T. Nozawa, F. Maruyama, K. Tsumoto, S. Hamada, I. Nakagawa
  • Journal Title: Cellular Microbiology (in press)
  • Peer Reviewed
• [Presentation] レンサ球菌属ゲノム情報に基づく種特異的進化機構の解析 (2010)
  • Author(s): 丸山史人
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] ビブリオ科細菌における薬剤耐性プラスミド全塩基配列解析とその伝達機構 (2009)
  • Author(s): 野中里佐・丸山史人・宮本学・黒川顕・増田道明
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-21
• [Presentation] 次世代シーケンサーの基礎と細菌学への応用 (2009)
  • Author(s): 丸山史人
  • Organizer: 第3回細菌学・若手コロセウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-26
• [Presentation] 芳香族化合物による土壌撹乱に対するバクテリア群集の環境応答のメタゲノム解析 (2009)
  • Author(s): 森宙史, 加藤広海, 堂園亜由美, 大坪嘉行, 宮腰昌利, 丸山史人, 豊田敦, 藤山秋佐夫, 永田裕二, 黒川顕, 津田雅孝
  • Organizer: 細菌学若手コロッセウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-26
• [Presentation] Species-specific evolutionary strategies revealed from genome and transcriptome of Streptococcus pyogenes and S. mutans (2009)
  • Author(s): F. Maruyama, H. Mori, C. Aikawa, T. Nozawa, K. Kurokawa, I. Nakagawa
  • Organizer: ProkaGENOMICS 2009
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2009-10-04
• [Presentation] A visualization tool for comparative community analyses using nearest relatives (2009)
  • Author(s): H. Mori, F. Maruyama, K. Kurokawa
  • Organizer: ProkaGENOMICS 2009
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2009-10-04
• [Presentation] 口腔領域レンサ球菌による宿主上皮細胞への付着侵入と炎症の誘導 (2009)
  • Author(s): 桜井敦朗, 岡橋暢夫, 中田匡宣, 寺尾豊, 丸山史人, 川端重忠, 大嶋隆
  • Organizer: 第18回Lancefieldレンサ球菌研究会
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-26
• [Presentation] Streptococcus mutansおよびS. pyogenesゲノム・トランスクリプトームからみた種分化機構の解明 (2009)
  • Author(s): 丸山史人, 大野雅幸, 相川知広, 野澤孝志, 桜井敦朗, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] A群レンサ球菌の全ゲノム発現解析に基づく細胞内生存機構の解明 (2009)
  • Author(s): 大野雅幸, 丸山史人, 相川知広, 野澤孝志, 桜井敦朗, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] オートファジー誘導によるA群レンサ球菌感染上皮の細胞死制御機構の解析 (2009)
  • Author(s): 相川知広, 野澤孝志, 大野雅幸, 丸山史人, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] A群レンサ球菌感染時にオートファジーを誘導するNLRファミリーと相互作用する宿主因子の機能解析 (2009)
  • Author(s): 野澤孝志, 桜井敦朗, 大野雅幸, 相川知広, 丸山史人, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] Streptococcus mutansおよびS. pyogenesゲノム・トランスクリプトームからみた種分化機構の解明 (2009)
  • Author(s): 丸山史人, 大野雅幸, 相川知広, 野澤孝志, 桜井敦朗, 中川一路
  • Organizer: ゲノム微生物学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05