IDENTIFICATION OF KEY FACTORS IN CISPLATIN-INDUCED NEPHROTOXICITY BY MICROARRAY ANALYSIS USING THE ISOLATED RENAL TUBULES

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790133
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: YONEZAWA Atsushi Kyoto University, 医学研究科, 助教 (90452341)
• Project Period (FY): 2008 – 2009
• Keywords: 医療薬剤学 / 薬学 / マイクロアレイ / トランスポータ / 腎臓 / シスプラチン

Research Abstract

Kidney consists of several renal segments. To clarify the gene expression profile in the renal tubule, which is the origin of cisplatin-induced nephrotoxicity, microarray analysis using the isolated tubules was carried out. At first, we confirmed that this experimental method reflected the tubular gene expression. In the isolated tubules of cisplatin-treated rats, the gene expression of chemokines was elevated. Chemokines are suggested to be the key factors of the renal tubular genes in cisplatin-induced nephrotoxicity.

Research Products

• [Journal Article] Heterozygous variants of multidrug and toxin extrusions (MATE1 and MATE2-K) have little influence on the disposition of metformin in diabetic patients. (2010)
  • Author(s): Toyama K, Yonezawa A, Tsuda M, Masuda S, Yano I, Terada T, Osawa R, Katsura T, Hosokawa M, Fujimoto S, Inagaki N, Inui K
  • Journal Title: Pharmacogenet Genomics. 20(2) Pages: 135-138
  • Peer Reviewed
• [Journal Article] Transcellular transport of organic cations in double-transfected MDCK cells expressing human organic cation transporters hOCT1/hMATE1 and hOCT2/hMATE1. (2008)
  • Author(s): Sato T, Masuda S, Yonezawa A, Tanihara Y, Katsura T, Inui K.
  • Journal Title: Biochem Pharmacol. 76(7) Pages: 894-903
  • Peer Reviewed
• [Journal Article] Identification and Functional Characterization of a Novel Human and Rat Riboflavin Transporter, RFT1. (2008)
  • Author(s): Yonezawa A, Masuda S, Katsura T, Inui K.
  • Journal Title: Am J Physiol Cell Physiol 295(3) Pages: C632-641
  • Peer Reviewed
• [Presentation] 単離近位尿細管を用いたマイクロアレイ解析:シスプラチン腎症増悪を反映する分子の探索 (2010)
  • Author(s): 小澤愛子
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] Comparative functional characteristics of novel human riboflavin transporter, hRFT1 and hRFT2. (2009)
  • Author(s): Yoshiaki Yao
  • Organizer: 24th JSSX Annual Meeting
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-11-27
• [Presentation] Effect of multidrug and toxin extrusion (MATE1 and MATE2-K) polymorphisms on the disposition of metformin. (2009)
  • Author(s): Kana Toyama
  • Organizer: 24th JSSX Annual Meeting
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-11-27
• [Presentation] 腎mRNA発現データベースを活用した新規リボフラビントランスポータRFT1の同定、シンポジウム「尿細管輸送体調節と疾病 (2009)
  • Author(s): 米澤淳
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-04
• [Presentation] 腎排泄型カチオン性薬物メトホルミンのクリアランス予測因子の探索 (2009)
  • Author(s): 遠山佳奈
  • Organizer: 日本薬剤学会第24年会
  • Place of Presentation: 静岡
  • Year and Date: 2009-05-21
• [Presentation] IDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OF NOVEL MAMMALIAN RIBOFLAVIN TRANSPORTER RFT1. (2009)
  • Author(s): Atsushi Yonezawa
  • Organizer: 3rd Asian Pacific Regional ISSX Meeting
  • Place of Presentation: Bangkok, Thai
  • Year and Date: 2009-05-10
• [Presentation] 腎有機カチオン輸送系におけるメトホルミンとシメチジンの薬物間相互作用機構の解明 (2009)
  • Author(s): 橋本真弥
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] IDENTIFICATION OF HUMAN AND RAT NOVEL RIBOFLAVIN TRANSPORTER RFT1 BASED ON THE IN SILICO GENE EXPRESSION DATABASE. (2008)
  • Author(s): Atsushi Yonezawa
  • Organizer: 日本薬物動態学会第23回年会
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-30