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2009 Fiscal Year Final Research Report

The development of anti-tuberculosis gene drug inducing autophagy and activation of macrophages

Research Project

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Project/Area Number 20790145
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Medical pharmacy
Research InstitutionTokyo University of Science

Principal Investigator

FUJIWARA Naruyoshi  Tokyo University of Science, 薬学部, 助教 (50365425)

Project Period (FY) 2008 – 2009
Keywords医療薬剤学
Research Abstract

Tuberculosis, which is caused by Mycobacterium tuberculosis (MTB), is one of the most frequently occurring infections in the world. MTB is an intracellular parasitic bacterium that mainly infects the respiratory tract and hides in alveolar macrophages (AMs). Recently, it was reported that induction of autophagy in macrophages eliminates MTB1). In this study, we tried to develop a gene drug for inducing autophagy and activating macrophages. The genes, which related to atophagy and macrophage activation were first cloned by PCR and recombined into the expression vector (CAT7 neo). Each gene was then transfected to mouse macrophage cell line, RAW267.4 by using an Amaxa system. After introducing genes, the effects of these gene drug candidates were examined. The induction of autophagy in macrophages and activation of macrophages by some of our gene drugs were confirmed by fluorescent microscopic observation and western blot analysis. Based on these findings, it was shown that the gene drugs were effective for MTB. At present, the side effects such as inflammation by introducing gene drugs into macrophages are being estimated and the other candidates for gene drugs which induce autophagy and activation of macrophages are being screened.
1) Gutierrez, M.G.. et al. (2004). Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macro- phages. Cell 119, 753-766.

  • Research Products

    (2 results)

All 2010 2009

All Journal Article (1 results) Presentation (1 results)

  • [Journal Article] Colloids and Surface B2010

    • Author(s)
      T. Onoshita, N. Fujiwara, et al.
    • Journal Title

      Biointerfaces 76

      Pages: 151-157

  • [Presentation] The development of anti-tuberculosis gene drug inducing autophagy and activating of macriphage2009

    • Author(s)
      Naruyoshi Fujiwara, et al.
    • Organizer
      日本薬物動態学会第2 4回年会
    • Place of Presentation
      (ポスター)
    • Year and Date
      20091100

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

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