Functional analysis of miRNA dependent cisplatin resistance mechanisms.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Meiji Pharmaceutical University
• Principal Investigator: SUZUKI Toshihiro Meiji Pharmaceutical University, 薬学部, 講師 (80322527)
• Project Period (FY): 2008 – 2009
• Keywords: シスプラチン / miRNA / 薬剤耐性 / バイオマーカー

Research Abstract

Cisplatin is an effective anticancer agent that is widely used in the treatment of cancer. However, the development of resistance to cisplatin is a major obstacle to treatment. To understand the clinically relevant mechanisms of resistance, many studies have been aimed at clarifying the biochemical/molecular alterations of cisplatin-resistance cells. However, these studies did not conclusively identify the basis of cellular resistance to cisplatin.
In this study, micro RNA expression profiles of cisplatin resistant cell lines were analyzed using microarray and real time RT-PCR. We found some of micro RNAs were similarly regulated in couple of cisplatin resistant non small lung cancer cell lines. These finding were useful information for understanding of the miRNA mediated cisplatin resistance mechanisms.

Research Products

• [Journal Article] Role of multidrug resistance- associated protein 1 in the pathogenesis of allergic airway inflammation (2009)
  • Author(s): Yoshioka M, Sagara H, Takahashi F, Harada N, Nishio K, Mori A, Ushio H, Shimizu K, Okada T, Ota M, Ito YM, Nagashima O, Atsuta R, Suzuki T, Fukuda T, Fukuchi Y, Takahashi K
  • Journal Title: Am. J. Physiol. Lung Cell Mol. Physiol. 296 Pages: 30-36
  • Peer Reviewed
• [Journal Article] Profiling SLCO and SLC22 genes in the NCI-60 cancer cell lines to identify drug uptake transporters (2008)
  • Author(s): Okabe M, Szakacs G, Reimers MA, Suzuki T, Hall MD, Abe T, Weinstein JN, Gottesman MM
  • Journal Title: Mol Cancer Ther. 9 Pages: 3081-3091
• [Presentation] Kazuto Nishio, Tadayasu Togawa, Kanako Sugawara, Hitoshi Sakuraba, Analysis of miRNA mediated Cisplatin resistant mechanisms. (2010)
  • Author(s): Toshihiro Suzuki
  • Organizer: Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Washington DC
  • Year and Date: 20100400
• [Presentation] シスプラチン耐性肺癌細胞株におけるmiRNA発現プロファイル (2009)
  • Author(s): 鈴木俊宏、西尾和人、兎川忠靖、菅原佳奈子、櫻庭均
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20091000
• [Presentation] シスプラチン耐性肺癌細胞株におけるmiRNAの発現解析 (2009)
  • Author(s): 鈴木俊宏、西尾和人、兎川忠靖、菅原佳奈子、櫻庭均
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091000
• [Presentation] 耐性細胞を用いたシスプラチン耐性因子の探索 (2009)
  • Author(s): 鈴木俊宏、兎川忠靖、菅原佳奈子、西尾和人、姫野誠一朗、櫻庭均
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 20090300
• [Presentation] 薬剤耐性における金属輸送の役割 (2008)
  • Author(s): 鈴木俊宏、兎川忠靖、櫻庭均
  • Organizer: 第1回メタロミクス研究フォーラムS5-4
  • Place of Presentation: 東京
  • Year and Date: 20081200
• [Presentation] 耐性細胞を用いたシスプラチン耐性因子の探索 (2008)
  • Author(s): 鈴木俊宏、兎川忠靖、西尾和人、姫野誠一郎、櫻庭均
  • Organizer: 第81回日本生化学会大会/第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20081200
• [Presentation] 発現クローニングによるシスプラチン耐性因子の探索 (2008)
  • Author(s): 鈴木俊宏、Shen Ding-Wu、岡部光規、兎川忠靖、Gottesman M Michael、櫻庭均
  • Organizer: がん分子標的治療研究会
  • Place of Presentation: 東京
  • Year and Date: 20080600
• [Presentation] シスプラチンの耐性と金属輸送機構 (2008)
  • Author(s): 鈴木俊宏
  • Organizer: 日本薬学会第128年会シンポジウムS24
  • Place of Presentation: 横浜
  • Year and Date: 20080300