2010 Fiscal Year Final Research Report
Study of side effect reduction of anticancer agents by regulation of exogenous gene expression
| Project/Area Number |
20790152
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Himeji Dokkyo University |
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Principal Investigator |
KINOSHITA Atsushi Himeji Dokkyo University, 薬学部, 講師 (60454766)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 医療薬剤学 / 遺伝子治療 |
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| Research Abstract |
For the purpose of regulation of transgene expression after gene introduction in gene therapy, the levels of CMV-promoter-driven transgene expression was analyzed when transgene introduced cells was exposed by anticancer agents. The levels of CMV-promoter-driven transgene expression were increased by doxorubicin (Dox) exposure. The induction of CMV-promoter-driven transgene expression by Dox was decreased by co-treatment of edaravone. We also found that phosphorylation pathway of AP-1 having CMV-promoter binding ability was activated by Dox.
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