Reincorporation of cathepsin D in neurons of mouse brain.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790162
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Niigata University
• Principal Investigator: SHIBATA Masahiro Niigata University, 医歯学系, 准教授 (10343253)
• Project Period (FY): 2008 – 2009
• Keywords: リソソーム / カテプシン / ライソゾーム病 / セロイドリポフスチノーシス

Research Abstract

Central nervous system specific cathepsin D (CD) deficient mice (CD:NesCre) ied around P40 by seizures and their phenotype was similar to neuronal ceroid lipofuscinosis. We noticed that CD protein was detected in neurons of CD:NesCre mice by immunohistochemistry, although CD mRNA was not expressed in the neurons. These results indicate that CD:NesCre neurons receive CD from other type of cells. We have also developed the chick experimental model to examine the transport system of lysosomal enzymes and established the stable expression system of transgenes into chick genome.

Research Products

• [Journal Article] Autophagic neuron death (2009)
  • Author(s): Uchiyama Y, Koike M, Shibata M, Sasaki M.
  • Journal Title: Methods Enzymol 453 Pages: 33-51
• [Journal Article] Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation (2009)
  • Author(s): Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, Dono K, Doki Y, Kominami E, Sugioka A, Monden M
  • Journal Title: Uchiyama Y. Autophagy 5(3) Pages: 351-60
  • Peer Reviewed
• [Journal Article] Autophagic neuron death in neonatal brain ischemia/hypoxia (2008)
  • Author(s): Uchiyama Y, Koike M, Shibata M.
  • Journal Title: Autophagy 4(4) Pages: 404-8
  • Peer Reviewed
• [Presentation] 鶏胚での条件的遺伝子発現抑制法 (2010)
  • Author(s): 柴田昌宏、佐藤昇
  • Organizer: 第115回日本解剖学会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 2010-03-28
• [Presentation] Efficient gene transfer to developing chick astrocytes by Tol2 transposition (2009)
  • Author(s): M. Shibata, R. Inoue, N. Sato
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] Lysosomal cathepsin D in neurons is in part delivered from glial cells (2008)
  • Author(s): M. Shibata, M. Koike, Y. Uchiyama
  • Organizer: 第38回米国神経科学会
  • Place of Presentation: ワシントンDC、米国
  • Year and Date: 2008-11-17
• [Presentation] Reincorporation of cathepsin D in neurons of mouse brain (2008)
  • Author(s): M. Shibata, M. Koike, Y. Uchiyama
  • Organizer: 第34回日本神経科学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-09