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2009 Fiscal Year Final Research Report

A cell adhesion molecule close homologue of L1 increased in primary afferent terminal contributes to the development and maintenance of neuropathic pain

Research Project

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Project/Area Number 20790170
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHyogo College of Medicine

Principal Investigator

YAMANAKA Hiroki  Hyogo College of Medicine, 医学部, 講師 (20340995)

Project Period (FY) 2008 – 2009
Keywords神経因性疼痛 / 細胞間接着 / 脊髄後角 / シナプス / 形態変化
Research Abstract

The L1 family of cell adhesion molecules (L1-CAMs) is known to regulate various neural functions that are pivotal to nervous system, including cell adhesion, axon guidance and synaptic plasticity. We investigated the involvement of a close homologue of the L1 cell adhesion molecule (CHL1) on neuropathic pain produced in the rat spared nerve injury (SNI) model. SNI induced the expression of CHL1 in L4/5 DRG neurons, particularly in small size injured neurons and in satellite cells. In the spinal cord, CHL1-immunoreactivity increased in laminae I-II of the dorsal horn ipsilateral to the injury. Ultrastructural study clarified the localization of CHL1 in the axon of primary afferents in the ipsilateral dorsal horn. CHL1 immunoreactivites were localized in the adherence such as axon- axon, axon-dorsal horn neurons (dendrite, soma) and axon-glia (astrocyte and microglia). Experimental inhibition of CHL1 adhesion by chronic intrathecal administration of the anti-CHL1 extracellular domain significantly prevented andreversed SNI-induced mechanical allodynia. Thus, alterations of CHL1 may be involved in the structural plasticity that is associated with neuropathic pain.

  • Research Products

    (22 results)

All 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (16 results)

  • [Journal Article] Comparative study of the distribution of the alpha-subunits of voltage-gated sodium channels in normal and axotomized rat dorsal root ganglion neurons.2008

    • Author(s)
      Fukuoka, T., Kobayashi, K., Yamanaka, H., Obata, K., Dai, Y., Noguchi, K.
    • Journal Title

      J. Comp. Neurol. 510

      Pages: 188-206

    • Peer Reviewed
  • [Journal Article] Transforming growth factor-activated kinase 1 induced in spinal astrocytes contributes to mechanical hypersensitivity after nerve injury.2008

    • Author(s)
      Katsura, H., Obata, K., Miyoshi, K., Kondo, T., Yamanaka, H., Kobayashi, K., Dai, Y., Fukuoka, T., Sakagami, M., Noguchi, K.
    • Journal Title

      Glia 56

      Pages: 723-733

    • Peer Reviewed
  • [Journal Article] P2Y12 receptor upregulation in activated microglia is a gateway of p38 signaling and neuropathic pain.2008

    • Author(s)
      Kobayashi, K., Yamanaka, H., Fukuoka, T., Dai, Y., Obata, K., Noguchi, K.
    • Journal Title

      J. Neurosci. 28

      Pages: 2892-2902

    • Peer Reviewed
  • [Journal Article] Phospholipase C and protein kinase A mediate bradykinin sensitization of TRPA1: a molecular mechanism of inflammatory pain.2008

    • Author(s)
      Wang, S., Dai, Y., Fukuoka, T., Yamanaka, H., Kobayashi, K., Obata, K., Cui, X., Tominaga, M., Noguchi, K.
    • Journal Title

      Brain 131

      Pages: 1241-1251

    • Peer Reviewed
  • [Journal Article] Activation of extracellular signal-regulated protein kinase in sensory neurons after noxious gastric distention and its involvement in acute visceral pain in rats.2008

    • Author(s)
      Sakurai, J., Obata, K., Ozaki, N., Tokunaga, A., Kobayashi, K., Yamanaka, H., Dai, Y., Kondo, T., Miyoshi, K., Sugiura, Y., Matsumoto, T., Miwa, H., Noguchi, K.
    • Journal Title

      Gastroenterology 134

      Pages: 1094-1103

    • Peer Reviewed
  • [Journal Article] Differential activation of p38 and extracellular signal-regulated kinase in spinal cord in a model of bee venom-induced inflammation and hyperalgesia.2008

    • Author(s)
      Cui, X.Y., Dai, Y., Wang, S.L., Yamanaka, H., Kobayashi, K., Obata, K., Chen, J., Noguchi, K.
    • Journal Title

      Mol. Pain 4

      Pages: 17

    • Peer Reviewed
  • [Presentation] Transient expression of TNF alpha in the rat spinal cord following peripheral nerve iujury.2009

    • Author(s)
      Kobayashi K, Yamanaka, H., Okubo M, Dai Y, Noguchi K.
    • Organizer
      The 39th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Chicago, U.S.A.
    • Year and Date
      20091017-20091021
  • [Presentation] Platelet-activating factor biosynthesis in spinal cord contributes to neuropathic pain following peripheral nerve injury.2009

    • Author(s)
      Okubo M, Yamanaka, H., Kobayashi K, Noguchi K.
    • Organizer
      The 39th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Chicago, U.S.A.
    • Year and Date
      20091017-20091021
  • [Presentation] 末梢神経損傷後に脊髄で増加したロイコトリエンは神経因性疼痛に関与する2009

    • Author(s)
      大久保正道, 山中博樹, 小林希実子, 野口光一
    • Organizer
      第31回日本疼痛学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090717-20090718
  • [Presentation] 腰部交感神経節ニューロンにおける電位依存性ナトリウムチャネルの発現と軸索切断後の変化2009

    • Author(s)
      福岡哲男, 小林希実子, 山中博樹, 野口光一
    • Organizer
      第31回日本疼痛学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090717-20090718
  • [Presentation] 末梢神経損傷後に脊髄マイクログリアで増加するp2y受容体2009

    • Author(s)
      小林希実子, 山中博樹, 大久保正道, 野口光一
    • Organizer
      第31回日本疼痛学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090717-20090718
  • [Presentation] 末梢神経損傷後に脊髄で増加するP2Y受容体2009

    • Author(s)
      小林希実子, 山中博樹, 野口光一
    • Organizer
      第47回日本生物物理学会
    • Place of Presentation
      徳島
    • Year and Date
      2009-11-01
  • [Presentation] ラット坐骨神経障害モデルにおけるERMの発現とリン酸化2009

    • Author(s)
      樫本良亮, 山中博樹, 小林希実子, 大久保正道, 三村治, 野口光一
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-18
  • [Presentation] 末梢神経損傷後に脊髄で合成が増加した血小板活性化因子は神経因性疼痛に関与する2009

    • Author(s)
      大久保正道, 山中博樹, 小林希実子, 野口光一
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-18
  • [Presentation] 末梢神経損傷後の脊髄後角マイクログリアで増加するp2受容体2009

    • Author(s)
      小林希実子, 山中博樹, 大久保正道, 野口光一
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-18
  • [Presentation] Induction of annexin A2 in rat dorsal root ganglia and spinal cord following peripheral nerve injury2008

    • Author(s)
      Yamanaka, H., Okubo, M., Kobayashi, K., Noguchi, K.
    • Organizer
      The 38th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C., U.S.A.
    • Year and Date
      20081115-20081119
  • [Presentation] Induction of P2Y receptor in the spinal cord following peripheral nerve injury2008

    • Author(s)
      Kobayashi, K., Yamanaka, H., Fukuoka, T., Noguchi, K.
    • Organizer
      The 38th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C., U.S.A.
    • Year and Date
      20081115-20081119
  • [Presentation] Laminae-specific distribution of Nav mRNAs in the rat spinal cord2008

    • Author(s)
      Fukuoka, T., Kobayashi, K., Yamanaka, H., Obata, K., Dai, Y., Noguchi, K.
    • Organizer
      The 38th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C., U.S.A.
    • Year and Date
      20081115-20081119
  • [Presentation] Upregulation of leukotriene synthesis in glial cells contributes to neuropathic pain following peripheral nerve injury2008

    • Author(s)
      Okubo, M., Yamanaka, H., Kobayashi, K., Noguchi, K.
    • Organizer
      The 38th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C., U.S.A.
    • Year and Date
      20081115-20081119
  • [Presentation] 一次知覚ニューロンにおける電位依存性ナトリウムチャネルサブタイプの発現比較2008

    • Author(s)
      福岡哲男, 小林希実子, 山中博樹, 戴毅, 小畑浩一, 野口光一
    • Organizer
      第30回日本疼痛学会
    • Place of Presentation
      福岡
    • Year and Date
      20080719-20080720
  • [Presentation] 皮膚と後根神経節におけるロイコトリエン合成酵素および受容体の発現2008

    • Author(s)
      大久保正道, 山中博樹, 小林希実子, 野口光一
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Year and Date
      20080709-20080711
  • [Presentation] ラット後根神経節におけるロイコトリエン受容体の発現2008

    • Author(s)
      大久保正道, 山中博樹, 野口光一
    • Organizer
      第57回(社)全日本鍼灸学会学術大会(京都大会)
    • Place of Presentation
      京都
    • Year and Date
      20080530-20080601

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Published: 2011-06-18   Modified: 2016-04-21  

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