2009 Fiscal Year Final Research Report
Analysis of molecules related to structure and function of T-tubule in cardiac myocytes
| Project/Area Number |
20790174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Shinshu University |
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Principal Investigator |
NAKADA Tsutomu Shinshu University, 医学部, 助教 (70452141)
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| Project Period (FY) |
2008 – 2009
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| Keywords | T管 / 循環器 / 心筋 |
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| Research Abstract |
Transverse-tubule (T-tubule) is invagination of plasma membrane, only found in ventricular and skeletal myocytes, and have important role for excitation and contraction coupling. To identify molecules involved in formation and maintain of T-tubule structure in cardiac myocyte, mRNA expression of candidate genes were determined by Northern blotting. Among the genes tested, Pacsin3, a member of F-BAR protein family, was highly expressed in adult ventricular myocyte. To reveal the functions of Pacsin3, several deletion mutants was transiently transfected in COS7 cell. C-terminal deletion mutant of Pacsin3 was co-localized with alpha-tubulin, and changed form of microtubule network.
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