Molecular mechanisms for cell migration

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790225
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Nagoya University
• Principal Investigator: WATANABE Takashi Nagoya University, 高等研究院, 特任講師 (10402562)
• Project Period (FY): 2008 – 2009
• Keywords: 細胞生物学 / 生化学 / 細胞医化学

Research Abstract

Directional cell migration is orchestrated cellular process that is required for a variety of physiological processes such as embryogenesis, wound healing, angiogenesis, as well as carcinogenesis. Migrating cells show a distinct polarity with forming front-rear axis toward the direction. In this study, focusing on Rho family GTPases that is critical regulator in polarized migration, we aimed to elucidate the molecular mechanisms governing migratory behavior and cell polarity.

Research Products

• [Journal Article] Identification of focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3-kinase) as Par3 partners by proteomic analysis. (2010)
  • Author(s): Itoh, N., Nakayama, M., Nishimura, T., Fujisue, S., Nishioka, T., Watanabe, T., Kaibuchi, K.
  • Journal Title: Cytoskeleton (Hoboken) 67 Pages: 297-308
  • Peer Reviewed
• [Journal Article] The PHCCEx domain of Tiam1/2 is a novel protein- and membrane-binding module. (2010)
  • Author(s): Terawaki, S., Kitano, K., Mori, T., Zhai, Y., Higuchi, Y., Itoh, N., Watanabe, T., Kaibuchi, K., Hakoshima, T.
  • Journal Title: EMBO J 29 Pages: 236-250
  • Peer Reviewed
• [Journal Article] Phosphorylation of CLASP2 by GSK-3beta regulates its interaction with IQGAP1, EB1 and microtubules. (2009)
  • Author(s): Watanabe, T., Noritake, J., Kakeno, M., Matsui, T., Harada, T., Wang, S., Itoh, N., Sato, K., Matsuzawa, K., Iwamatsu, A., et al.
  • Journal Title: J Cell Sci 122 Pages: 2969-2979
  • Peer Reviewed
• [Journal Article] TDP-43 depletion induces neuronal cell damage through dysregulation of Rho family GTPases. (2009)
  • Author(s): Iguchi, Y., Katsuno, M., Niwa, J., Yamada, S., Sone, J., Waza, M., Adachi, H., Tanaka, F., Nagata, K., Arimura, N., Watanabe, T., et al.
  • Journal Title: J Biol Chem 284 Pages: 22059-22066
  • Peer Reviewed
• [Journal Article] Ultrastructural study of Rac1 and its effectors beneath the substratum-facing membrane. (2008)
  • Author(s): Watanabe, T., Wang, S., Kakeno, M., Usukura, J., Kaibuchi, K.
  • Journal Title: Cell Struct Funct 33 Pages: 101-107
  • Peer Reviewed
• [Journal Article] Prognostic implication and biological roles of RhoH in acute myeloid leukaemia. (2008)
  • Author(s): Iwasaki, T., Katsumi, A., Kiyoi, H., Tanizaki, R., Ishikawa, Y., Ozeki, K., Kobayashi, M., Abe, A., Matsushita, T., Watanabe, T., et al.
  • Journal Title: Eur J Haematol 81 Pages: 454-460
  • Peer Reviewed
• [Journal Article] Mutational analysis of SOS1 gene in acute myeloid leukemia. (2008)
  • Author(s): Tanizaki, R., Katsumi, A., Kiyoi, H., Kunishima, S., Iwasaki, T., Ishikawa, Y., Kobayashi, M., Abe, A., Matsushita, T., Watanabe, T., et al.
  • Journal Title: Int J Hematol 88 Pages: 460-462
  • Peer Reviewed
• [Presentation] In vitro reconstitution of microtubule dynamics in cell cortex. (2009)
  • Author(s): Watanabe, T., Kakeno, M., Wang, S., Matsuzawa, K., Sato, K., Kaibuchi K.
  • Organizer: Gordon Research Conference.
  • Place of Presentation: Oxford, UK
  • Year and Date: 20090826-20090827
• [Presentation] Role of Par and Tiam1 complex in polarized cell migration. (2009)
  • Author(s): Watanabe, T., Wang, S., Matsuzawa, K., Sato, K., Kakeno, M., Kaibuchi K.
  • Organizer: The 32nd Annual meeting of the Molecular Biology Society of Japan.
  • Place of Presentation: Yokohama
  • Year and Date: 2009-12-12
• [Presentation] Targeting of Tiam1 to focal adhesion through Talin for Rac1 activation and cell migration. (2009)
  • Author(s): Watanabe, T., Wang, S., Matsuzawa, K., Sato, K., Kakeno, M., Kaibuchi K.
  • Organizer: 49th Annual Meeting for American Society for Cell Biology
  • Place of Presentation: San Diego, CA
  • Year and Date: 2009-12-07
• [Presentation] Role of IQGAP1 in regulation of microtubule dynamics in polarized cell migrating cells. (2009)
  • Author(s): Watanabe, T., Kakeno, M., Wang, S., Matsuzawa, K., Sato, K., Kaibuchi K.
  • Organizer: The 61st Annual Meeting of the Japan Society for Cell Biology.
  • Place of Presentation: Nagoya
  • Year and Date: 2009-06-03
• [Presentation] Asymmetric Targeting of Tiam1 to Focal Adhesion through Talin for Polarized Cell Migration. (2009)
  • Author(s): Watanab, T., Wang, S., Matsuzawa, K., Sato K., Kakeno M., Kaibuchi, K.
  • Organizer: DB symposim Shape and Polarity.
  • Place of Presentation: Kobe
  • Year and Date: 2009-03-24
• [Presentation] Roles of the Rho family GTPases and IQGAP1 in Cell migration. (2008)
  • Author(s): Watanabe, T., Kaibuchi, K.
  • Organizer: BMB2008
  • Place of Presentation: Kobe
  • Year and Date: 2008-12-10
• [Book] Cadherin-mediated Intercellular Adhesion and Signaling Cascades Involving Small GTPases.(Cold Spring Harb Perspect Biol, 1) (2009)
  • Author(s): Watanabe, T., Sato, K., Kaibuchi, K.
  • Total Pages: a003020