2009 Fiscal Year Final Research Report
Mass production of enucleated erythrocyte from erythroid progenitor cell lines.
| Project/Area Number |
20790240
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
HIROYAMA Takashi The Institute of Physical and Chemical Research, 細胞材料開発室, 専任研究員 (50373361)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Keywords | ES / iPS / 赤血球 / 細胞株 / MEDEP / WAVE2 / Rac / ROCK |
|---|
| Research Abstract |
In this study, we developed a method that enables to produce enucleated erythrocyte efficiently from mouse erythroid progenitor cell lines (MEDEP). And it was indicated that erythroid membrane protein, GPC and Rac-WAVE2 signaling pathway have important rolls in enucleation process. In addition, a culture method using a ROCK inhibitor, by which hematopoietic cells can be induced from human ES or iPS cells, was developed. However, human erythroid progenitor cell line has not been established.
|
