Adaptation of the most suitable anticancer drug by utilizing new developed three-dimensional model mimicked human tumor microenvironment.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790295
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: TSUDA Masumi Hokkaido University, 大学院・医学研究科, 助教 (30431307)
• Project Period (FY): 2008 – 2009
• Keywords: 腫瘍微小環境 / 抗がん剤

Research Abstract

We identify receptor activator of nuclear factor κB ligand (RANKL) as a microenvironment-specific factor essential for tumorigenesis in vivo, utilizing oral squamous cell carcinoma as a model. In addition, in human synovial sarcoma that frequently occurs pulmonary metastasis, the interaction between the tumor and endothelial cells is important for tumor angiogenesis. Src family kinase inhibitor impaired this angiogenesis effectively.

Research Products

• [Journal Article] The adaptor protein Crk induces Src-dependent activation of p38 MAPK in regulation of synovial sarcoma cell proliferation. (2009)
  • Author(s): T.Watanabe, †M.Tsuda, S.Tanaka, Y.Ohba, H.Kawaguchi, T.Majima, H.Sawa, A.Minami
  • Journal Title: Mol.Cancer Res. 7(9) Pages: 1582-92
  • Peer Reviewed
• [Journal Article] Crk adaptor protein induced-phosphorylation of Gab1 on tyrosine 307 via Src is important for organization of focal adhesions and enhanced cell migration. (2009)
  • Author(s): T.Watanabe, M.Tsuda, Y.Makino, T.Konstantinou, H.Nishihara, T.Majima, A.Minami, S.Feller, S.Tanaka
  • Journal Title: Cell Res. 19(5) Pages: 638-650
  • Peer Reviewed
• [Journal Article] Integral role of transcription factor 8 in the negative regulation of tumor angiogenesis. (2009)
  • Author(s): T.Inuzuka, M.Tsuda, H.Kawaguchi, Y.Higashi, Y.Ohba
  • Journal Title: Cancer Res. 69(4) Pages: 1678-1684
  • Peer Reviewed
• [Presentation] RANKL expression induced by the tumor microenvironment promotes epithelial mesenchymal transition and tumor progression in vivo (2010)
  • Author(s): Yamada T, Tsuda M, Kawaguchi H, Totsuka Y, Shindoh M, Ohba Y
  • Organizer: The 8th Joint Conference of the American Association for Cancer Research and the Japanese Cancer Association
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20100205-20100209
• [Presentation] Effect of Src family kinase inhibitor on proliferation and invasion in human synovial sarcoma in vivo. (2009)
  • Author(s): Arai R, Tsuda M, Watanabe T, Kawaguchi H, Minami A, Ohba Y
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Requirement for tumor microenvironment-induced RANKL expression in the tumororigenesis in vivo. (2009)
  • Author(s): Yamada T, Tsuda M, Totsuka Y, Shindoh M, Ohba, Y
  • Organizer: 第68回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20091001-20091003
• [Presentation] Development of synovial sarcoma-like tumors in SYT-SSX1 transgenic p53 mutant mice. (2008)
  • Author(s): Tsuda M, Watanabe T, Ohba Y, Tanaka S
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20081028-20081030
• [Remarks]
  • URL: http://www.med.hokudai.ac.jp/~clilab-w/