The role of c-KIT positive cancer cells in a drug resistance of the intestinal tumors

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790303
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: KITAMURA Takanori Kyoto University, 医学研究科, 助教 (10378622)
• Project Period (FY): 2008 – 2009
• Keywords: 腫瘍・マウスモデル・大腸癌 / c-KIT / 抗癌剤

Research Abstract

Apc/Smad4 compound mutant mice develop intestinal adenocarcinomas with marked tumor invasion. We demonstrated here that cancer cells at the invasion fronts expressed a progenitor cell marker c-KIT. Although 5-fluorouracil treatments retracted the invasive adenocarcinomas, the c-KIT positive cancer cells did not disappeared from the tumors in contrast to the c-KIT negative cells. These results suggest that the c-KIT positive cells are resistant to 5-fluorouracil treatments.

Research Products

• [Journal Article] Induction and down-regulation of Sox17 and its possible roles during the course of gastrointestinal tumorigenesis. (2009)
  • Author(s): Du YC, Oshima H, Oguma K, K itamura T, Itadani H, Fujimura T, Piao YS, Yoshimoto T, Minamoto T, Kotani H, Taketo MM, Oshima M
  • Journal Title: Gastroenterology 137(4) Pages: 1346-1357
  • Peer Reviewed
• [Journal Article] Reduced level of smoothened suppresses intestinal tumorigenesis by down-regulation of Wne signaling. (2009)
  • Author(s): Arimura S, Matsunaga A, Kitamura T, Aoki K, Aoki M, Taketo MM.
  • Journal Title: Gastroenterology 137(2) Pages: 629-638
  • Peer Reviewed
• [Journal Article] Matrix metalloproteinase 7 is required for tumor formation, but dispensable for invasion and fibrosis in SMAD4-deficient intestinal adenocarcinomas. (2009)
  • Author(s): Kitamura T, Biyajima K, Aoki M, Oshima M, Taketo MM
  • Journal Title: Lab Invest 89(1) Pages: 98-105
  • Peer Reviewed
• [Journal Article] Inactivation of CCR1 suppresses colon cancer liver metastasis by blocking accumulation of immature myeloid cells in a mouse model.
  • Author(s): Kitamura T, Fujishita T, Loetscherb P, Reveszb L, Hashidac H, Kizaka-Kondohd S, Aoki M, Taketo MM
  • Journal Title: Proc Natl Acad Sci USA (In press)
  • Peer Reviewed
• [Presentation] The role of CCR1-expressing myeloid cells in invasion and metastasis of colon cancer cells (2009)
  • Author(s): 北村剛規、藤下晃章、武藤誠
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-10-22
• [Presentation] 腸癌の浸潤・転移における骨髄由来 CCR1発現細胞の役割 (2009)
  • Author(s): 北村剛規、藤下晃章、武藤誠
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Remarks]
  • URL: http://www4.mfour.med.kyoto-u.ac.jp/