2009 Fiscal Year Final Research Report
Research of the mechanism that occludin regulate microvilli formation and fence function in epithelial cells
| Project/Area Number |
20790308
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MURATA Masaki Sapporo Medical University, 医学部, 助教 (10404592)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 病理学 / 組織・細胞 / シグナル伝達 / タイト結合 / 上皮極性 |
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| Research Abstract |
Expression of occludin induces microvilli formation in epithelial cells. But there is no change in the expression of the molecules associated with microvilli (ezrin, radixin, moesin, and EBP-50) at protein and mRNA level. Occludin regulates the localization of ezrin by controlling the phosphorylation of ezrin in this time. In addition, a similar change is seen when introducing only C-terminal cytoplasmic domain of occludin is transfected in non-epithelial cells. Occludin may play an important role in the formation of microvilli in epithelial cells by phosphorylation of molecules associated with microvilli via the C-terminal cytoplasmic domain.
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