Studies on molecular factors responsible for active growth of Echinococcus multilocularis metacestodes

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790321
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nihon University
• Principal Investigator: MATUMOTO Jun Nihon University, 生物資源科学部, 講師 (70296169)
• Project Period (FY): 2008 – 2009
• Keywords: 人獣共通寄生虫 / エキノコックス

Research Abstract

This study aims to elucidate molecular factors responsible for the active growth of Echinococcus multilocularis metacestodes. We established a full-length cDNA library of the parasite by V-capping method. In silico analyses of this cDNA library containing over 20,000 clones revealed expression profile in the larval parasite. We are presently developing a E. multilocularis DNA microarray, which is expected to facilitate comprehensive expression analyses of the parasite. Experimental infections of larval E. multilocularis to 4 inbred mouse strainsidentified DBA/2 and C57BL/10 strains as highly susceptible and resistant strains, respectively. Based on these findings in this study, we are performing further genetic experiments using backcrossing mice with various genetic backgrounds originated from the resistant and susceptible mouse strains.

Research Products

• [Journal Article] Echinococcus multilocularis: Two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs. (2010)
  • Author(s): Kouguchi, H., Matsumoto, J., Katoh, Y., Suzuki, T., Oku, Y., Yagi, K.
  • Journal Title: Experimental Parasitology 124(2) Pages: 238-243
• [Journal Article] Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. (2009)
  • Author(s): Dang, Z., Yagi, K., Oku, Y., Kouguchi, H., Kajino, K., Watanabe, J., Matsumoto, J., Nakao, R., Wakaguri, H., Toyoda, A., Sugimoto, C.
  • Journal Title: Vaccine 27(52) Pages: 7339-7345
• [Journal Article] Molecular cloning and characterization of a T24-like protein in Echinococcus multilocularis. (2009)
  • Author(s): Dang, Z., Watanabe, J., Kajino, K., Oku, Y., Matsumoto, J., Yagi, K., Kouguchi, H., Sugimoto, C.
  • Journal Title: Molecular and Biochemical Parasitology 168(1) Pages: 117-119
• [Journal Article] Cutaneous leishmaniasis caused by Leishmania (L. ) major infection in Sindh province, Pakistan. (2009)
  • Author(s): Bhutto, A.M., Soomro, F.R., Baloch, J.H., Matsumoto, J., Uezato, H., Hashiguchi, Y., Katakura, K.
  • Journal Title: Acta Tropica 111(3) Pages: 295-298
• [Presentation] 多包虫のcDNAライブラリーに見られた抗酸化活性関連遺伝子. (2009)
  • Author(s): 平松澪、水上智秋、松本淳、野中成晃、片倉賢、李爽、山下理宇、渡辺純一、奥祐三郎
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 20090300