2009 Fiscal Year Final Research Report
Analysis of intestinal barrier disruption by HA proteins of botulinum toxin
| Project/Area Number |
20790336
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUMURA Takuhiro Osaka University, 微生物病研究所, 特任研究員 (00456930)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 細菌 / ボツリヌス菌 / ボツリヌス毒素 / 腸管上皮細胞間バリア |
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| Research Abstract |
In this study, we analyzed the mechanism of disruption of epithelial paracellular barrier function caused by botulinum HA. Using recombinant HA, we purified HA-binding proteins from human intestinal epithelial cell lines. Next, we analyzed the localization of botulinum toxins in vivo . The HA bound and transcytosed to the basolateral side and disrupted the epithelial barrier at the specific cells.
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