Regulation of virulence gene expression and genomic rearrangement by a nucleoid-associated protein Hha

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790348
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: YAMAMOTO Shouji National Institute of Infectious Diseases, 細菌第一部, 研究員 (80469957)
• Project Period (FY): 2008 – 2009
• Keywords: 病原因子 / 遺伝子発現 / 遺伝子再編成 / 核様体蛋白質

Research Abstract

This study was done to understand the regulatory mechanism of virulence gene expression by the Hha and GrlA proteins in enterohemorrhagic Escherichia coli (EHEC). This study showed that : 1)the loss of Hha suppressed the ability of GrlA to activate virulence gene expression, 2)GrlA had no effect on the expression of Hha, and 3)GrlA did not interact with Hha. These results suggested that GrlA might regulate Hha activity through unidentified factor(s).

Research Products

• [Journal Article] LrhA positively controls the expression of the locus of enterocyte effacement genes in enterohemorrhagic Escherichia coli by differential regulation of their master regulators PchA and PchB. (2009)
  • Author(s): Honda N., Iyoda S., Yama mo to S., Terajima J., Watanabe H.
  • Journal Title: Mol. Microbiol. 74 Pages: 1393-1411
• [Journal Article] Application ofλRed recombination system to Vibrio cholerae genetics: Simple methods for inactivation and modification of chromosomal genes. (2009)
  • Author(s): Yam am ot o S., Izumiya H., Morita M., Arakawa E., Watanabe H.
  • Journal Title: Gene 438 Pages: 57-64
• [Journal Article] Transcription of the ehx enterohemolysin gene is positively regulated by GrlA, a global regulator encoded within the locus of enterocyte effacement in enterohemorrhagic Escherichia coli. (2008)
  • Author(s): Saitoh T., Iyoda S., Yamam ot o S., Lu Y., Shimuta K., Ohnishi M., Terajima J., Watanabe H.
  • Journal Title: J. Bacteriol. 190 Pages: 4822-4830
• [Journal Article] Appearance and genetic diversity of El Tor Vibrio cholerae O1 that possess classical biotype ctxB among imported cases cholera in Japan.
  • Author(s): Motita M., Ohnishi M., Arakawa E., Yam am ot o S., Matsushita S., Yokoyama K., Kai A., Seto K., Watanabe H., Izumiya H.
  • Journal Title: J. Med. Microbiol. (in press.)
• [Journal Article] Chitin disaccharide (GlcNAc)2 induces natural competence in Vibrio cholerae through transcriptional and translational activation of a positive regulatory gene tfoXVC.
  • Author(s): Yama mo to S., Morita M., Izumiya H., Watanabe H.
  • Journal Title: Gene (in press.)