2009 Fiscal Year Final Research Report
Visualization of influenza virus progeny RNP complex and analysis of its nucleus-to-plasma membrane transport mechanisms.
| Project/Area Number |
20790358
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kitasato University |
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Principal Investigator |
MOMOSE Fumitaka Kitasato University, 大学院・感染制御科学府, 講師 (90332204)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 共焦点レーザー顕微鏡 / インフルエンザウイルス / 微小管 / 宿主因子 / モノクローナル抗体 / ライブセルイメージング / タンパク質トランスフェクション |
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| Research Abstract |
Influenza virus genome is replicated in the nucleus as ribonucleoprotein complex (vRNP) and is transported to the cell surface where the budding of progeny virion occurs. This research revealed that the trafficking of progeny vRNP depends on the host cellular microtubule transport system. Live cell imaging of progeny vRNP demonstrated that the vRNP signals moved along microtubules rapidly (the average velocity of 0.7-1.5μm/s) but intermittently in both plus and minus directions.
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