M cell differentiation based on lympho-epithelial interaction

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790383
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HASE Koji The Institute of Physical and Chemical Research, 免疫系構築研究チーム, 研究員 (20359714)
• Project Period (FY): 2008 – 2009
• Keywords: M細胞 / パイエル板 / 上皮細胞 / B細胞 / 粘膜免疫

Research Abstract

M cells are developed in a course of cell-cell interaction between FAE and certain unidentified immune cells. We found that a unique B-cell subset characterized by CCR6^<hi>CD11c^+ resided in mouse PP.Adoptive transfer of CCR6^<hi>CD11c^+ B cells from wild-type mice restored the M-cell decrement in CCR6-deficient mice. Collectively, the spatial regulation of CCR6^<hi>CD11c^+ B cells via the CCL20-CCR6 system may play a vital role in M-cell differentiation in mice.

Research Products

• [Journal Article] Uptake through glycoprotein 2 of FimH(+) bacteria by M cells initiates mucosal immune response. (2009)
  • Author(s): Hase K, Kawano K, Nochi T, Pontes GS, Fukuda S, Ebisawa M, Kadokura K, Tobe T, Fujimura Y, Kawano S, Yabashi A, Waguri S, Nakato G, Kimura S, Murakami T, Iimura M, Hamura K, Fukuoka S, Lowe AW, Itoh K, Kiyono H, Ohno H.
  • Journal Title: Nature 462 Pages: 226-30
  • Peer Reviewed
• [Journal Article] M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex. (2009)
  • Author(s): Hase K, Kimura S, Takatsu H, Ohmae M, Kawano S, Kitamura H, Ito M, Watarai H, Hazelett CC, Yeaman C, Ohno H.
  • Journal Title: Nat. Cell Biol. 11 Pages: 1427-32
  • Peer Reviewed
• [Journal Article] New approach for M-cell-specific molecules screening by comprehensive transcriptome analysis. (2009)
  • Author(s): Nakato G, Fukuda S, Hase K, Goitsuka R, Cooper MD, Ohno H.
  • Journal Title: DNA Res. 16 Pages: 227-235
  • Peer Reviewed
• [Journal Article] Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease. (2008)
  • Author(s): Hase K, Takahashi D, Ebisawa M, Kawano S, Itoh K, Ohno H.
  • Journal Title: PLoS ONE 3 Pages: e3033
  • Peer Reviewed
• [Journal Article] A novel subset of mouse NKT cells bearing the IL-17 receptor B responds to IL-25 and contributes to airway hyperreactivity. (2008)
  • Author(s): Terashima A, Watarai H, Inoue S, Sekine E, Nakagawa R, Hase K, Iwamura C, Nakajima H, Nakayama T, TaniguchiM
  • Journal Title: J. Exp. Med. 205 Pages: 2727-33
  • Peer Reviewed
• [Journal Article] IL-6-dependent mucosal protection prevents establishment of a microbial niche for attaching/effacing lesion-forming enteric bacterial pathogens. (2008)
  • Author(s): Dann SM, Spehlmann ME, Hammond DC, Iimura M, Hase K, Choi LJ, Hanson E, Eckmann L.
  • Journal Title: J. Immunol 180 Pages: 6816-26
  • Peer Reviewed
• [Journal Article] Comprehensive gene expression profiling of Peyer's patch M cells, villous M-like cells, and intestinal epithelial cells. (2008)
  • Author(s): Terahara K, Yoshida M, Igarashi O, Nochi T, Pontes GS, Hase K, Ohno H, Kurokawa S, Mejima M, Takayama N, Yuki Y, Lowe AW, Kiyono H.
  • Journal Title: J. Immunol 180 Pages: 7840-6
  • Peer Reviewed
• [Presentation] GP2-dependent antigen transcytosis by M cells initiates mucosal immune response. The 4th Stage Surface Barrier Immunology Study Group (SBARIS). (2010)
  • Author(s): Hase K
  • Organizer: 2nd Meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20100129-20100130
• [Presentation] GP2-dependent antigen transcytosis by M cells initiates mucosal immune response. (2009)
  • Author(s): Hase K
  • Organizer: 第39回日本免疫学会・国際シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 20091202-20091204
• [Presentation] Uptake via Glycoprotein 2 of FimH+ bacteria by M cells initiates mucosal immune response. (2009)
  • Author(s): Hase K, Kawano K, Nochi T, Pontes GS, Lowe AW, Kiyono H, Ohno H.
  • Organizer: 14th International Congress of Mucosal Immunology (ICMI2009)
  • Place of Presentation: Boston, USA
  • Year and Date: 20090705-20090709
• [Presentation] 粘膜免疫系の最前線におけるM細胞依存的抗原取り込み機構の解明 (2009)
  • Author(s): 長谷耕二
  • Organizer: 日本食品免疫学会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-05-27
• [Presentation] 腸管特殊上皮m細胞による抗原トランスサイトーシスの分子機構 (2008)
  • Author(s): 長谷耕二
  • Organizer: 第13回日本食物繊維学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20081121-20081122
• [Presentation] Function and differentiation of intestinal follicular M cells. (2008)
  • Author(s): Hase K
  • Organizer: The 3rd JSI-RCAI Immunology Workshop
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-14
• [Remarks]
  • URL: http://leib.rcai.riken.jp/riken/index.html